Zika Virus Methods and Protocols

Zika Virus: A Brief History and Review of its Pathogenesis Rediscovered.- Zika Virus Isolation, Purification, and Titration.- Evaluating Zika Virus Pathogenesis in Immunocompromised Mice.- Droplet Digital PCR and Immunohistochemistry Techniques to Detect Zika Virus in the Central Nervous System of Mice.- A Plaque Reduction Neutralization Test for the Detection of ZIKV-Specific Antibodies.- Analysis of Serum Anti-Zika Virus Antibodies by Focus Reduction Neutralization Test.- Enzyme-Linked Immunosorbent Assay and Quantitative Reverse Transcription PCR as a Technique to Analyze Inflammation.- Production and Purification of Zika Virus NS1 Glycoprotein in HEK293 Cells.- Optimization of Small-Scale Production of Zika Virus Envelope Glycoprotein by Transient Expression in HEK293 Cells for ELISA.- Zika Virus Peptide ELISA (ZIKV-NS2B-concat ELISA) for Detection of IgG Antibodies to Zika Virus Infection.- Zika Virus Amplification using Strand Displacement Isothermal Method and Sequencing using Nanopore Technology.- Detection of Zika Virus using Reverse Transcription-Loop-Mediated Isothermal Amplification (RT-LAMP).- Development and Validation of Real-Time RT-LAMP Assays for the Specific Detection of Zika Virus.- Comparative Analysis of Zika Virus Detection by RT-qPCR, RT-LAMP, and RT-RPA.- A Porcine Model of Zika Virus Infection to Profile the In Utero Interferon Alpha Response.- Isolation and Detection of Zika Virus-Infected Rhesus Macaques Lymph Node Cells and Splenocytes.- Discovery of Zika Virus Dependency and Restriction Factors using Flow-Based Arrayed CRISPR Screening for Identification of Targets (FACS-IT).- Zika Virus Targets Multiple Tissues and Cell Types During the First Trimester of Pregnancy.- Use of Primary Human Fetal Astrocytes and Tissue Explants as Ex Vivo Models to Study Zika Virus Infection of the Developing Brain.- Isolation of Monoclonal Antibodies from ZikaVirus-Infected Patient Samples.