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Virus Detection




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Dettagli

Genere:Libro
Lingua: Inglese
Editore:

CRC Press

Pubblicazione: 05/2023
Edizione: 1° edizione





Note Editore

Viruses do not behave as other microbes; their life cycles require infecting healthy cells, commandeering their cellular apparatus, replicating and then killing the host cell. Methods for virus detection and identification have been developed only in the past few decades. These recently developed methods include molecular, physical, and proteomic techniques. All these approaches (Electron Microscopy, Molecular, Direct Counting, and Mass Spectrometry Proteomics) to detection and identification are reviewed in this succinct volume. It is written in approachable language with enough detail for trained professionals to follow and want to recommend to others. Key Features Covers common detection methods Reviews the history of detection from antiquity to the present Documents the strengths and weaknesses of various detection methods Describes how to detect newly discovered viruses Recommends specific applications for clinical, hospital, environmental, and public health uses




Sommario

Abbreviations and Glossary7Preface8About the Author11Abstract14Virus Detection15Chapter 1 – Civilization and Disease15Chapter 2 – Microbes, Fungi, Bacteria, and Viruses442.1 Fungi472.1.1 What is a Fungus472.1.2 How are Fungi detected/classified472.2 Bacteria482.2.1. What are Bacteria?482.2.2 How are Bacteria detected and classified482.3 Viruses492.3.1 What are viruses?492.3.2 How are Viruses detected and classified50Chapter 3 - Indirect Methods of Detecting viruses63Chapter 4 - Electron microscopy.654.1 Transmission ission Electron microscopy674.1.1 How does a Tranmission Electron Microscope (TEM) work?674.1.2 How do you use Electron Microscopy684.1.3 How do you identify a new virus694.2 Scanning Electron microscopy744.3 Examples of both TEM and SEM visualization of viruses.77Chapter 5 - Molecular Methods for detecting viruses965.1 Polymerase Chain Reaction (PCR)985.2 Antibody Methods1135.3 How do you add new viruses to the Antibody Method of detection?115Chapter 6 - Direct Virus Counting methods, such as IVDS.1166.1 How does direct counting work?1176.2 How do you identify a new virus with direct counting?1186.3 Why IVDS was invented1186.4 Flow chart showing how to use IVDS for virus detection1206.5 The Recommended uses of IVDS1246.6 Improving sensitivity of IVDS (Concentration and Accumulation)1296.7 An Example - Following COV-19 through 5 days and then a 3 Month follow-up1336.8 PCR and IVDS Compared1476.9 Summary of the Fielded IVDS149Chapter 7 – Mass Spectrometry Proteomic (MSP) Method1507.1 Introduction1517.2 Ion Mobility and Various Types of Mass Analyzers1547.2.1 Using Electrospray Ionization (ESI) method in detecting viruses1557.3 How do MSP Methods work for biological detection?1577.4 Detection and Identification of Viruses using MSP1597.5 Examples of Viruses Detected by MSP Methods using ABOid1627.5.1 African Swine Fever Virus (ASFV) - Variola Porcina1637.5.2 Alcelaphine Herpesvirus 1 (AlHV-1)1657.5.3 CamelpoxVirus (CMLV)1657.5.4 Cercopithecine herpesvirus 5 (CeHV-5)1667.5.5 Goatpox Virus Pellor (GTPV)1667.5.6 Lumpy Skin Disease Virus (LSDV)1667.5.7 MonkeypoxVirus Zaire-96-I-16 (MPV)1677.5.8 Sheeppox Virus1687.5.9 Vaccinia Virus (VACV)1687.5.10 Variola Virus (VARV)1687.5.11 Discussion of Viruses Detected by MSP and ABOid1697.6 Adding new viruses using MSP1707.7 Coronavirus Detection including SARS.1757.7.1 Coronavirus Viruses1767.7.2 National Average for Coronavirus1777.7.8 Verifying COVID-19 detection.1787.7.9 COVID-19 Detection Discussion1807.8 Summary of MSP-ABOid detection of viruses180Chapter 8 - Discussion1828.1 Introduction1848.2 What are the challenges when detecting viruses?1918.3 Clinical1948.4 Environmental1978.5 Using during a Pandemic2018.6 Other uses203References208




Autore

Charles H. Wick is a retired lieutenant colonel and senior scientist from the U.S. Army Edgewood Chemical Biological Center. He earned his PhD from the University of Washington. Dr. Wick is best known for work in forensic science done in concert with the Department of Defense. Dr. Wick’s research work has resulted in international recognition as an authority on individual performance for operations conducted on a nuclear, biological, and chemical battlefield. He has made lasting and important contributions to forensic science and antiterrorism and holds several U.S. patents in the area of microbe detection and classification. He has written more than 45 civilian and military publications and has received various awards and citations.










Altre Informazioni

ISBN:

9780367617981

Condizione: Nuovo
Collana: Pocket Guides to Biomedical Sciences
Dimensioni: Ø 1.00 lb
Formato: Brossura
Illustration Notes:53 b/w images, 12 color images, 15 halftones, 38 line drawings and 12 color line drawings
Pagine Arabe: 136
Pagine Romane: xxii


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