Virus-Derived Nanoparticles for Advanced Technologies Methods and Protocols

This volume details protocols on virus-derived nanoparticles (VNPs) for a number of different applications. Chapters guide readers through the production of  VNPs derived from plant, animal and bacterial viruses, prokaryotic and eukaryotic expression systems,  encapsulation of heterologous materials within VNPs, and the modification of the outer surface of VNPs and how such modified VNPs can be developed into functional entities. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

 Authoritative and practical, Virus-Derived Nanoparticles for Advanced Technologies: Methods and Protocols aims to ensure successful results in the further study of this vital field.

 The Production in Plants of Mosaic Turnip Crinkle Virus-like Particles Derived by Co-infiltration of Wild-Type and Modified Forms of Virus Coat Protein.- Isolation and Characterization of Two Distinct Types of Unmodified Spherical Plant Sobemovirus-like Particles for Diagnostic and Technical Uses.- RNA-directed Assembly of Tobacco Mosaic Virus (TMV)-Like Carriers with Tunable Fractions of Differently Addressable Coat Proteins.- Fabrication of Tobacco Mosaic Virus-like Nanorods for Peptide Display.- In planta Production of Fluorescent Filamentous Plant Virus-based Nanoparticles.- Self-assembling Plant-derived Vaccines against Papillomaviruses.- Recombinant Expression of Tandem-HBc Virus-like Particles (VLPs).- Production and Application of Insect Virus-based VLPs.- Nanomanufacture of Free-standing, Porous, Janus-Type Films of Polymer-Plant Virus Nanoparticle Arrays.- Self-assembly of Rod-like Bionanoparticles at Interfaces and in Solution.- Bottom-up Assembly of TMV-based Nucleoprotein Architectures on Solid Supports.- Internal Deposition of Cobalt Metal and Iron Oxide within CPMV eVLPs.- Plant Virus-based Nanoparticles for the Delivery of Agronomic Compounds as a Suspension Concentrate.- Nanowires and Nanoparticle Chains inside Tubular Viral Template.- In vitro-Reassembled Plant Virus-Like Particles of Hibiscus Chlorotic Ringspot Virus (HCRSV) as Nano-protein Cages for Drugs.- CCMV-based Enzymatic Nanoreactors.- Protocol for the Efficient Cell-free Synthesis of Cowpea Chlorotic Mottle Virus-like Particles Containing Heterologous RNAs.- Packaging DNA Origami into Viral Protein Cages.- In Vitro Assembly of Virus-Derived Designer Shells around Inorganic Nanoparticles.- In Vivo Packaging of Protein Cargo inside of Virus-Like Particle P22.- Encapsulation of Negatively Charged Cargo in MS2 Viral Capsids.- Delivering Cargo: Plant-based Production of Bluetongue Virus core- and Virus-like Particles Containing Fluorescent Proteins.- Bioinspired Silica Mineralization on Viral Templates.- Propagation and Isolation of Tobacco Mosaic Virus Which Surface Displays Metal Binding and Reducing Peptides for Generation of Gold Nanoparticles.- TMV-templated Formation of Metal and Polymer Nanotubes.- Semiconducting Hybrid Layer Fabrication Scaffolded by Virus Shells.- Dual Functionalization of Rod-Shaped Viruses on Single Coat Protein Subunits.- Drug-loaded Plant-virus Based Nanoparticles for Cancer Drug Delivery.- Construction of Artificial Enzymes on a Virus Surface.- Redox-Immuno Functionalized Potyvirus Nanoparticles for High Resolution Imaging by AFM-SECM Correlative Microscopy.- Presenting Peptides at the Surface of Potyviruses in planta.- Engineering of M13 Bacteriophage for Development of Tissue Engineering Materials.- Displaying Whole-chain Proteins on Hepatitis B virus Capsid-like Particles.- Dual-functionalized Virus-gold Nanoparticles Clusters for Biosensing.- TMV-based Adapter Templates for Enhanced Enzyme Loading in Biosensor Applications.- Integrated Methods to Manufacture Hydrogel Microparticles Containing Viral-metal Nanocomplexes with High Catalytic Activity.- Integrated Methods to Manufacture Hydrogel Microparticles with High Protein Conjugation Capacity and Binding Kinetics via Viral Nanotemplate Display.- Interactions between Plant Viral Nanoparticles (VNPs) and Blood Plasma Proteins, and their Impact on the VNP In Vivo Fates.- Fabrication of Plant Virus- based Thin Films to Modulate the Osteogenic Differentiation of Mesenchymal Stem Cells.- Dual Surface Modification of Genome-free MS2 Capsids for Delivery Applications.- Virus-Based Cancer Therapeutics for Targeted Photodynamic Therapy.