Vaccinia Virus Methods and Protocols

This detailed book provides practical information for the laboratory that can be applied to the study of vaccinia and other poxviruses while emphasizing long-standing field standards and focusing on emerging new technologies applied in the field of poxvirology. The methods and protocols have been designed with the bench scientist in mind, being presented in a fashion that makes them useful for both starting and veteran poxvirus researchers. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

1-Laboratory safety and vaccinia (Isaacs)

 

2-Preparation and purification of VACV MVs and EVs

 

3-CRISPR mediated poxvirus genome editing (Yaohe Wang)

 

4-Vaccinia and ribosome profiling (Yang)

 

5-Pox bioinformatics (Sobhy)

 

6-High-through Image analyses of infection

 

7-RNAi against VACV genome proteins

 

8-Construction of recombinant VACVs (transient expressors/tagged)

 

9-Transient complementation of deletions with stable expressing cell lines (Weibe)

 

10- Super-resolution imaging of poxviruses (Albrect/Gray)

 

11-Vaccinia virus RNA sequencing (Yang Z or Moss)

 

12-Vaccinia antibody production (Cohen)

 

13-HTS for VACV cell targets (Mercer)

 

14-Drug screening against poxviruses (Hruby)

 

15-VACV DNA preparation for molecular biology cloning (Roper)

 

16-Transient complementation assays for mutant protein assessment (traktman)

 

17-VACV in vitro transcription from purified cores (broyles)

 

18-Studying vaccinia replication (Traktman)

 

19-Quatitaive PCR assessment of VACV genome copies (Moss)

 

20-Click-chemistry and genome labeling (Kilcher/Mok)

 

21-Vaccinia infection mouse models (buller)

 

22-Fluoresent recombinant VACV and live cell imaging (Newsome)

 

23-VACV bioluminescence imaging

 

24-VACV in vivo intravital imaging (Hickman Yewdell)

 

25-Pox and oncolytics (Mcfadden)