Type-1 Diabetes Methods and Protocols

This volumes details methods focusing on technological innovation and recent advances in diabetes management. Chapters will guide readers through recent advances, beta-cell regeneration, non-invasive imaging of endogenous, transplanted islets theranostics, microRNA profiling of beta-cells and artificial intelligence, and deep learning algorithms in diabetes. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.

3D optical molecular imaging of the rodent pancreas by OPT and LSFM.- Uncommon Transplantation Sites – Transplantation of islets and islet organoids in the anterior chamber of the eye of rodents and monkeys.- Generation of Insulin-producing multicellular organoids.- Non-invasive quantitative PET Imaging in humans of the Pancreatic Beta Cell Mass biomarkers VMAT2 and Dopamine, D2/D3 receptors, in vivo.- In Vivo Imaging of Naked and Microencapsulated Islet Cell Transplantation.- Diabetes retinopathy: new ways to detect and treat.- MRI methods for imaging beta-cell function in the rodent pancreas.- MicroRNAs in type 2 diabetes – focus on microRNA profiling in Islets of Langerhans.- Protocol for clinical GLP-1 receptor PET/CT imaging with [⁶⁸Ga]Ga-NODAGA-exendin-4.- Hyperinsulinemic-euglycemic clamp in conscious rats based on the tail artery and vein catheterization.- Synthesis of siRNA-conjugated dextran-coated iron oxide nanoparticles for islet protection during transplantation and noninvasive imaging.- Micelle-Based Nanocarriers for Targeted Delivery of Cargo to Pancreas.- Magnetic particle imaging of transplanted human islets using a machine learning algorithm.- In vivo bioluminescence for the detection of the fate of pancreatic islet organoids post-transplantation.- miRNA theranostic nanoparticles promote pancreatic beta cell proliferation in type 1 diabetes model.- Isolation and purification of human pancreatic islets.- Vascularized islet transplantation as composite Islet-Kidney grafts with nanoparticle-labeled islets in large animal preclinical transplant models.