Type-1 Diabetes Methods and Protocols

This volumes details methods focusing on technological innovation and recent advances in diabetes management. Chapters will guide readers through recent advances, beta-cell regeneration, non-invasive imaging of endogenous, transplanted islets theranostics, microRNA profiling of beta-cells and artificial intelligence, and deep learning algorithms in diabetes. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.

1. 3D optical molecular imaging of the rodent pancreas by OPT and LSFM

Max Hahn and Ulf Ahlgren

3. Uncommon Transplantation Sites – Transplantation of islets and islet organoids in the anterior chamber of the eye of rodents and monkeys

Tilo Moede, Sai Bo Bo Tun, Ingo B. Leibiger, and Per-Olof Berggren

3. Generation of Insulin-producing multicellular organoids

Laura Mar-Fonseca, Fanny Lebreton, Charles-Henri Wassmer, and Ekaterine Berishvili

4. Non-invasive quantitative PET Imaging in humans of the Pancreatic Beta Cell Mass biomarkers VMAT2 and Dopamine, D2/D3 receptors, in vivo.

5. In Vivo Imaging of Naked and Microencapsulated

Islet Cell Transplantation

Dian R. Arifin and Jeff W.M. Bulte

6. Diabetes retinopathy: new ways to detect and treat

Todd A. Lydic and Julia V. Busik

7. MRI methods for imaging beta-cell function in the rodent pancreas

Pooyan Khalighinejad, Eul Hyun Suh, and A. Dean Sherry

8. MicroRNAs in type 2 diabetes – focus on microRNA profiling in Islets of Langerhans

Elaine Cowan, Alexandros Karagiannopoulos, Lena Eliasson

9. Protocol for clinical GLP-1 receptor PET/CT imaging with [⁶⁸Ga]Ga-NODAGA-exendin-4

S. Tokgöz, M. Boss, S. Prasad, P. Shah, P. Laverman, M. van Riel, and M. Gotthardt

10. Hyperinsulinemic-euglycemic clamp in conscious rats based on the tail artery and vein catheterization

Xiaohuan Liu, LuLu Chen, and Xiang Hu

11. Synthesis of siRNA-conjugated dextran-coated iron oxide nanoparticles for islet protection during transplantation and noninvasive imaging.

Neil Robertson, Ping Wang, Nazanin Talebloo Kazuhiko Yamada, and Anna Moore

12. Micelle-Based Nanocarriers for Targeted Delivery of Cargo to Pancreas

Swati Mishra and Philip R. Streeter

13. Magnetic particle imaging of transplanted human islets using a machine learning algorithm

Aixia Sun, Hasaan Hayat, Simon W. Sanchez, Anna Moore, and Ping Wang 

14. In vivo bioluminescence for the detection of the fate of pancreatic islet organoids post-transplantation

Aixia Sun, Elizabeth Kenyon, Mithil Gudi, Wen Li, Aitor Aguirre, and Ping Wang

15. miRNA theranostic nanoparticles promote pancreatic beta cell proliferation in type 1 diabetes model

Saumya Nigam, Anna Moore, and Ping Wang

LingLing Wei

17. Vascularized islet transplantation as composite Islet-Kidney grafts with nanoparticle-labeled islets in large animal preclinical transplant models

Kazuhiko Yamada, Daniel L. Eisenson, Xiaojuan Chen, Lei Ji, Michelle R. Santillan, and Anna Moore