Tissue Proteomics Methods and Protocols

This volume details established workflows for biological interrogations to understand proteomics methods. Chapters guide readers through strategies for bottom-up tissue proteomics, proteomics landscape through different tissue types, proper decision tree for the tissue proteomics, nuanced approaches in tissue proteomics, and emerging research topics in targeted tissue proteomics. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and cutting-edge, Tissue Proteomics: Methods and Protocols aims to serve as a valuable resource to inspire new discoveries in the dynamic field of tissue proteomics.

Immunoaffinity Depletion of High-Abundance Proteins from Serum/Plasma for Proteomic Analysis.- Optimal Sample Preparation Workflow for Quantitative Mass Spectrometry-Based Studies in the Low Range.- In-Gel and In-Solution Approaches to Protein and Peptide Fractionation.- Protein Sample Preparation for Bottom-Up, Label-Free Quantitative Proteomics of Adipose Tissue.- Sample preparation and processing for quick, universal, and insightful microbial proteomics.- Proteomic Analysis of Single Hairs.- Shotgun Proteomics Protocol for Insects.- Insect Brain Proteomics – A Case Study of Periplanata americana.- Bottom-Up Proteomics Workflow for Studying Multi-Organism Systems.- Proteomic Analysis of Biological Fluids.- Analyzing Various Biological Fluids with a Single Automated Proteomic Workflow for Biomarker Discovery.- A Multi-Enzyme Protocol Improves Total Proteome Coverage in Extracellular Matrix.- A Protocol to Disclose the Protein Fingerprint of Commercial White Wines Based on Proteomic Tools.- Isolating Synaptic Vesicles from Neurospheres for Proteomics.- Isolation and Identification of Brain Tissue Extracellular Vesicles for Translational Proteomics.- Cross-Linking Mass Spectrometry to Capture Protein Network Dynamics of Cell Membranome.- In-Depth Analysis of Tissue Phosphoproteomics with TiO2 Enrichment and Fractionation with High pH Reversed-Phase Chromatography.- Mass Spectrometry - Based Glycomics and Proteomics Profiling of On-Slide Digested Tissue from Complex Biological Samples.- Multiplexed and Multiomic Mass Spectrometry Imaging of MALDI-IHC Photocleavable Mass-Tag Tissue Probes, N-Glycomics, and The Extracellular Matrisome from FFPE Tissue Sections.- Proteomics-Based Analysis of Laser-Capture Micro-Dissected, Formalin-Fixed Paraffin-Embedded Tissue Samples.- Decision Tree for Protein Biomarker Selection for Clinical Applications.- Computational Tools and Methods for the Study of Systemic Amyloidosis at the Clinical and Molecular Level.