Thyroid Hormones Methods and Protocols

This volume focuses on thyroid hormones and their transporters and receptors. Chapters focus on a wide range of methods and protocols that are important for advancing the elucidation of the role of thyroid hormones in our body. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.

Authoritative and cutting-edge, Thyroid Hormones: Methods and Protocols aims to be a useful and practical guide to new researchers and experts looking to expand their knowledge.

Thyroid hormones and co-workers: an overview.- Thyroid hormone action by genomic and nongenomic molecular mechanisms.- A sensitive radioimmunoassay for T3 and T4 determinations in plasma and tissues.- Generation of a mouse model for the study of thyroid hormones regulatory effect on the immune system.- Analysis of apoptosis by thyroid hormone induction.- Immunophenotyping of leukocytes in brain in hypothyroid mice.- Immunophenotyping of hematopoietic cells in the spleen in hypothyroid mice.- Mouse embryonic fibroblasts reprogramming to induced pluripotent stem cells by T3.- DNA methylation analysis by bisulfite pyrosequencing of mouse embryonic fibroblasts with reprogramming enhanced by thyroid hormones.- Analysis of the pattern of RNA expression in the skin of TR-deficient mice by RNA-seq.- miR-21, miR-31, miR-34a, and miR-203 changes validation by quantitative polymerase chain reaction in isolated keratinocytes.- Magnetic resonance imaging techniques for investigating the MCT8-deficient brain in murine disease models.- Review of surgical interventions in the thyroid gland: recent advances and current.- Immunohistochemical study protocol in thyroid pathology.- Thyroid hormones testing in euthyroid and LT4-treated patients during covid-19 hospitalization.