Preface…
Table of Contents…
Contributors…
Part I Isolation and Culture Methods
1. Isolation, Primary Culture, and Differentiation of Preadipocytes from Mouse Brown Adipose Tissue
Wei Gao, Xingxing Kong, and Qin Yang
2. Isolation of Mouse Stromal Vascular Cells for Monolayer Culture
Longhua Liu, Louise D. Zheng, Sarah R. Donnelly, Margo P. Emont, Jun Wu, and Zhiyong Cheng
3. Flow Cytometry Assisted Isolation of Adipose Tissue Derived Stem Cells
Umesh D. Wankhade and Sushil G. Rane
4. Flow Cytometric Isolation and Differentiation of Adipogenic Progenitor Cells into Brown and Brite/Beige Adipocytes
Jochen Steinbring, Antonia Graja, Anne-Marie Jank, and Tim J. Schulz
5. Immuno-Magnetic Isolation and Thermogenic Differentiation of White Adipose Tissue Progenitor Cells
Rohollah Babaeikelishomi, Irem Bayindir-Buchhalter, Irina Meln, and Alexandros Vegiopoulos
6. Isolation of Immune Cells from Adipose TissueJonathan R. Brestoff
7. Differentiation and Metabolic Interrogation of Human Adipocytes
Nicki A. Baker, Lindsey A. Muir, Carey N. Lumeng, and Robert W. O’Rourke
8. Protocols for Generation of Immortalized Human Brown and White Preadipocyte Cell Lines
Farnaz Shamsi and Yu-Hua Tseng
Part II Functional Analysis, Bioengineering, and Other Applications
9. Gene Expression and Histological Analysis of Activated Brown Adipocytes in Adipose Tissue
Yun-Hee Lee
10. Genetic Mouse Models: The Powerful Tools to Study Fat Tissues
Xingxing Kong, Kevin W. Williams, and Tiemin Liu
11. Genetic Manipulation with Viral Vectors to Assess Metabolism and Adipose Tissue Function
Nicolás Gómez-Banoy and James C. Lo
12. Bioenergetic Analyses in Adipose Tissue
Lawrence Kazak
13. Oxygen Consumption Rate and Energy Expenditure in Mice: Indirect Calorimetry
Eun Ran Kim and Qingchun Tong
14. Isolation and Patch-Clamp of Primary Adipocytes
Yanhui Zhang, Dan Tong, Anil Mishra, Litao Xie, and Rajan Sah
15. In Vitro Approaches to Model and Study Communications Between Adipose Tissue and the Liver
Sean O’Connor and Paul Cohen
16. Identification and Quantification of Human Brown Adipose Tissue
Maria Chondronikola, Craig Porter, John O. Ogunbileje, and Labros S. Sidossis
17. Designing 3D Adipospheres for Quantitative Metabolic Study
Takeshi Akama, Brendan M. Leung, Joe Labuz, Shuichi Takayama, and Tae-Hwa Chun
18. Culture and Sampling of Primary Adipose Tissue in Practical Microfluidic Systems
Jessica C. Brooks, Robert L. Judd, and Christopher J. Easley
19. Using Thermogenic Beige Cells to Identify Biologically Active Small Molecules and Peptides
Ling Wu and Bin Xu
