The Dynamic Synapse Molecular Methods in Ionotropic Receptor Biology

Bringing together the latest information into one easily accessible resource, The Dynamic Synapse: Molecular Methods in Ionotropic Receptor Biology explores the diverse tools and technologies used to study synaptic processes. Written by world-renowned leaders in the field, the book delineates newly developed techniques, methods, and conceptual advances used for studying neurotransmitter receptors and other synaptic proteins. Providing detailed information on cutting-edge techniques that can be applied to this area, the book is useful to both new and experienced synaptic physiologists as well as the wider neuroscience community.

Exploring the diverse tools and technologies used to study synaptic processes,The Dynamic Synapse: Molecular Methods in Ionotropic Receptor Biology delineatestechniques, methods, and conceptual advancesfor studying neurotransmitter receptors and other synaptic proteins. It describes abroadrange of molecular, biochemical, imaging, and electrophysiological approaches for studying the biology of synapses. Specific topics include the use of proteomics to study synaptic protein complexes, the development of phosphorylation state specific antibodies, post-genomic tools applied to the study of synapses and RNA interference in neurons. In addition, several chapters focus on methods for gene and protein delivery into neuronal tissue. The use of biochemical, electrophysiological and optical tagging techniques to study the movement and membrane trafficking of neurotransmitter receptors in the membrane of live nerve cells are also discussed. To complement these approaches, the application of approaches for achieving long-term alterations in the genetic complement of neurons in vivo using viral vectors or homologous recombination of ES cells are also described.

Methods for Uncovering the Mechanisms of AMPA Receptor Trafficking, S. Restituito and E.B. ZiffLong-Term Plasticity at Inhibitory Synapses: A Phenomenon That Has Been Overlooked, J.-L. Gaiarsa and Y. Ben-AriNew Tricks for an Old Dog: Proteomics of the PSD, B.A. Jordan, B.D. Fernholz, T.A. Neubert, and E.B. ZiffPhosphorylation Site-Specific Antibodies as Research Tools in Studies of Native GABAA Receptors, J.N. JovanovicProtein Palmitoylation by DHHC Protein Family, Y. Fukata, D.S. Bredt, and M. FukataStudying the Localization, Surface Stability and Endocytosis of Neurotransmitter Receptors by Antibody Labeling and Biotinylation Approaches, I.L. Arancibia-Cárcamo, B.P. Fairfax, S.J. Moss, and J.T. KittlerVisualization of AMPAR Trafficking and Surface Expression, P.V. Perestenko and J.M. HenleyNeurotransmitter Dynamics, S. Lévi and A. TrillerReceptor Dynamics at the Cell Surface Studied Using Functional Tagging, P. Thomas and T.G. SmartRNAi and Applications in Neurobiology, T.C. JacobTransfecting and Transducing Neurons with Synthetic Nucleic Acids and Biologically Active Macromolecules, J.T. Kittler, J.G. Hanley, and J.T.R. IsaacAcute In Vivo Expression of Recombinant Proteins in Rat Brain Using Sindbis Virus, H. Marie and R.C. MalenkaLentivirus-Based Genetic Manipulations in Neurons In Vivo, P. Osten, T. Dittgen, and P. LicznerskiAMPA Receptor Phosphorylation in Synaptic Plasticity: Insights from Knockin Mice, H.-K. LeeGenomic and Post-Genomic Tools for Studying Synapse Biology, J.T. Kittler and P.L. OliverIndex