Tertiary Lymphoid Structures Methods and Protocols

This second edition details new and updated chapters on technologies related to tertiary lymphoid structures( TLS). Chapters guide readers through TLS in cancers, different stages of TLS development, immune function of TLS, molecular, cellular, and imaging methods, and preclinical methods to uncover the function of cells composing TLS in inflammatory situations in humans. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.

Authoritative and cutting-edge, Tertiary Lymphoid Structures: Methods and Protocols, Second Edition aims to guide you through techniques and methods used to study the biology of TLS.

An introduction to tertiary lymphoid structures in cancer.- Tertiary lymphoid structures in central nervous system disorders.- Maturation of tertiary lymphoid structures.- Mouse Models Enable the Functional Investigation of Tertiary Lymphoid Structures in Cancer.- Home-built imaging-based spatial transcriptomics: Tertiary Lymphoid Structures as a case example.- Methods for selective gene expression profiling in single Tertiary Lymphoid Structure using Laser Capture Microdissection.- Laser Capture Microdissection of Tertiary Lymphoid Structures from Formalin-Fixed Paraffin-Embedded Sections of Canine Cutaneous and Subcutaneous Sarcomas for Nanostring direct RNA counting.- Comparative automated quantification of Tertiary Lymphoid Structures in two distinct mouse models of inflammation.- Imaging mass cytometry to decipher the maturation of tertiary lymphoid structures.- Advancement of techniques for precise visualization and quantification of tertiary lymphoid structure-associated immune cells in tissue samples.- Automated Quantification of Tertiary Lymphoid Structures in Human Tumor Samples Using Immunofluorescence and AI-Powered Analysis Pipeline.- Cancer-Associated Lymphoid Aggregates in Histology Images: Manual and Deep Learning-Based Quantification Approaches.- Method Development for Sorting Immune Cell Populations within Tertiary Lymphoid Structures.- Repertoires and tumor-reactivity analysis of B cell immunoglobulins derived from cancer patients.- Exploring the Role of Tertiary Lymphoid Structures using a mouse model of bacteria-infected lungs.- Tertiary Lymphoid Structures and Immunotherapy: Challenges and Opportunities.