T-Helper Cells Methods and Protocols

Human and Murine T helper Cell Recovery from Organs and Tissues.- Identification and Purification of Human Memory T Helper Cells from Peripheral Blood.- Transcription Factor Evaluation by Flow Cytometry.- Mass Cytometry Analysis of T Helper Cells.- Murine T Helper Cell Differentiation and Plasticity.- Identification of Follicular T Cell Subsets in Murine and Human Tissues.- Analysis of T Cells In Inflamed Non-Lymphoid Tissues.- TCR Vß Evaluation by Flow Cytometry.- Evaluation of Cytokine Production at the Single Cell Level by Flow Cytometry Upon Polyclonal Stimulation.- Evaluation of Secreted Cytokines by Multiplex Bead Based Assay (X MAP Technology, Luminex).- Quantifying Antigen-specific T Cells by Assessing their Antigen-Induced Proliferation.- Flow Cytometric Characterization of Human Antigen-Reactive T helper Cells.- Identification of Human Antigen-Specific CD4+ cells with Peptide-MHC Multimer Technologies.- Human Tcell Cloning by Limiting Dilution.- Human Germinal Center Reaction: Assessing T Follicular Helper-B cell Interactions In Vitro.- Study of Signal Transduction Pathways by Phospho-Protein Evaluation.- The Study of Protein-DNA Interactions in CD4+ T Cells using ChIPmentation.- mRNA Transfection of T lymphocytes by Electroporation.- Transduction of Human T Cell Subsets with Lentivirus.- Plasmid- or Ribonucleoprotein-mediated CRISPR/Cas Gene Editing in Primary Murine T Cells.- Generation of Sequencing Libraries for Building Immune Cell Methylomes.- Gene Expression Analysis of T Cells by Single Cell RNA-seq.- Quantifying Proteome and Protein Modifications in Activated T Cells by Multiplexed Isobaric Labeling Mass Spectrometry.- Metabolomics, Lipidomics and Immunometabolism.- Experimental Mouse Models of Asthma and Analysis of CD4+ T Cells.- Murine T Cell Transfer Colitis as a Model for Inflammatory Bowel Disease.- ExperimentalAutoimmune Encephalomyelitis.