Stem Cells in Regenerative Medicine

As regenerative medicine involves replacing diseased cells, tissues or organs, or repairing tissues in vivo, the manipulation of stem cells underlie its goals. In Stem Cells in Regenerative Medicine: Methods and Protocols, leading experts in the field provide an updated representation of the landscape of stem cell-based therapies in a wide spectrum of tissue systems and ontogenic stages, from the isolation and culture of stem cells to their actual use in vivo. Written in the highly successful Methods in Molecular Biology™ series format, these chapters include brief introductions to the topic, lists of the necessary materials and reagents, readily reproducible, step-by-step laboratory protocols, and tips for troubleshooting and avoiding known pitfalls.

Comprehensive and easy-to-use, Stem Cells in Regenerative Medicine: Methods and Protocols is certain to contribute greatly to the definition of standardized procedures for the manipulation of somatic and embryonic stem cells in research and clinical applications.

Stem Cell Sources and Manipulation.- Derivation and Manipulation of Murine Embryonic Stem Cells.- Patterning Mouse and Human Embryonic Stem Cells Using Micro-contact Printing.- Efficient Gene Knockdowns in Human Embryonic Stem Cells Using Lentiviral-Based RNAi.- Measurement of Cell-Penetrating Peptide-Mediated Transduction of Adult Hematopoietic Stem Cells.- Stem Cell Sources for Regenerative Medicine.- Regeneration of the Blood System.- Investigating the Interactions Between Haemopoietic Stem Cells and Their Niche: Methods for the Analysis of Stem Cell Homing and Distribution Within the Marrow Following Transplantation.- Ex Vivo Megakaryocyte Expansion and Platelet Production from Human Cord Blood Stem Cells.- Ex Vivo Generation of Human Red Blood Cells: A New Advance in Stem Cell Engineering.- Regeneration of the Nervous System.- Isolation and Manipulation of Mammalian Neural Stem Cells In Vitro.- Isolation, Expansion, and Differentiation of Mouse Skin-Derived Precursors.- Xenotransplantation of Embryonic Stem Cell-Derived Motor Neurons into the Developing Chick Spinal Cord.- Transplantation of Neural Stem/Progenitor Cells into Developing and Adult CNS.- Transplantation of Embryonic Stem Cell-Derived Dopaminergic Neurons in MPTP-Treated Monkeys.- Regeneration of the Epidermis.- Isolation and Culture of Epithelial Stem Cells.- Regeneration of Skin and Cornea by Tissue Engineering.- Regeneration of the Musculoskeletal System.- Prospective Isolation of Mesenchymal Stem Cells from Mouse Compact Bone.- Isolation, Propagation, and Characterization of Human Umbilical Cord Perivascular Cells (HUCPVCs).- Bone Marrow-Derived Mesenchymal Stem Cells: Isolation, Expansion, Characterization, Viral Transduction, and Production of Conditioned Medium.- Template DNA-Strand Co-Segregation and Asymmetric Cell Division in Skeletal Muscle Stem Cells.- Isolation and Grafting of Single Muscle Fibres.- Regeneration of the Vascular System.- Differentiation and Dynamic Analysis of Primitive Vessels from Embryonic Stem Cells.- Derivation of Contractile Smooth Muscle Cells from Embryonic Stem Cells.- Regeneration of the Pancreas and Liver.- Islet-Derived Progenitors as a Source of In Vitro Islet Regeneration.- Isolation and Characterization of Hepatic Stem Cells, or “Oval Cells,” from Rat Livers.- Reprogramming of Liver to Pancreas.