Spectral and Imaging Cytometry Methods and Protocols

This second edition volume expands on the previous edition with discussions on the latest approaches used in the spectral cytometry field. Beginning with a brief history of spectral cytometry development, this book continues with a section addressing new protocols in different areas of imaging cytometry based on the unique technology of Imagestream and also introduces FlowCam – cytometer with capabilities of analyzing phytoplankton and zooplankton. Written for the highly successful Methods in Molecular Biology series, chapters include brief introductions to their respective topics, complete lists of the materials and reagents necessary, and reproducible laboratory protocols with tips on troubleshooting and recommendations.

Chapters 1, 2, and 5 are available open access under a Creative Commons Attribution 4.0 International License via link.springer.com.

Development of Spectral Imaging Cytometry.- Using Virtual Filtering Approach to Discriminate Microalgae by Spectral Flow Cytometer.- Imaging Flow Cytometric Analysis of Primary Bone Marrow Erythroblastic Islands.- Imaging Flow Cytometry of Legionella-Containing Vacuoles in Intact and Homogenized Wild-Type and Mutant Dictyostelium.- Imaging Flow Cytometry of Multi-Nuclearity.- The Imaging Flow Cytometry-Based Cytokinesis-Block MicroNucleus (CBMN) Assay.- High-Throughput ?-H2AX Assay Using Imaging Flow Cytometry.- Label Free Identification of Persistent Particles in Association with Primary Immune Cells by Imaging Flow Cytometry.- “Immuno-FlowFISH": Applications for Chronic Lymphocytic Leukaemia.- Quantifying Golgi Apparatus Fragmentation Using Imaging Flow Cytometry.- Flow Imaging of the Inflammasome: Evaluating ASC Speck Characteristics and Caspase-1 Activity.- Quantitative Analysis of Latex Beads Phagocytosis by Human Macrophages Using Imaging Flow Cytometry with Extended Depth of Field (EDF).- FlowCam 8400 and FlowCam Cyano Phytoplankton Classification and Viability Staining by Imaging Flow Cytometry.- Optimizing FlowCam Imaging Flow Cytometry Operation for Classification and Quantification of Microcystis Morphospecies.