Single Cell Analysis Methods and Protocols

This volume explores the latest advancements and techniques used to study cell analysis, their capabilities, and the type of results that can be obtained. The chapters in this book cover topics such as FACS; fluorescence microscopy; organic spectroscopy such as MALDI; inorganic spectroscopy such as ICP-MS; and sequencing. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Cutting-edge and practical, Single Cell Analysis: Methods and Protocols is a valuable tool for any researcher interested in learning more about this important and developing field. 

Single Cell Isolation from Surgically Resected Tissue via Mechanical Dissociation using TissueGrinder,- Circulating Tumor Cell Enrichment and Single Cell Isolation Combining the CellSearch® and DEPArray™ Systems.- Isolation of Viable Epithelial and Mesenchymal Circulating Tumor Cells from Breast Cancer Patients.- Single Cell Recovery from Tumor Cell Xenotransplanted Zebrafish Embryos for the Study of Metastasis Initiating Cells.- Isolation of Single Circulating Tumor Cells using VyCAP Puncher System.- Simultaneous Isolation and Amplification of mRNA and Genomic DNA of a Single Cell.- Isolation and Genomic Analysis of Circulating Tumor Cell Clusters in Cancer Patients.- Establishing Single Cell Clones from In Vitro Cultured Circulating Tumor Cells.- Immunofluorescence Combined with Single-Molecule RNA Fluorescence In Situ Hybridization for Concurrent Detection of Proteins and Transcripts in Stress Granules.- Highly Multiplexed and Simultaneous Characterization of Protein and RNA in Single Cells by Flow or Mass Cytometry Platforms using Proximity Ligation Assay for RNA.- Array-Based Comparative Genomic Hybridization for the Detection of Copy Number Alterations in Single Cells.- Single Cell MicroRNA Sequencing Library Preparation.- Immunoblot Analysis from Single Cells using Milo™ Single-Cell Western Platform.- Imaging of Sub-Cellular Distribution of Platinum in Single Cells using Laser Ablation Inductively Coupled Plasma Mass Spectrometry.- Patch-seq: Multimodal Profiling of Single-Cell Morphology, Electrophysiology, and Gene Expression.- Single-Nucleus ATAC-seq for Mapping Chromatin Accessibility in Individual Cells of Murine Hearts.