Acknowledgement…
Preface…
Table of Contents…
Contributing Authors…
1. Single Cell Isolation from Surgically Resected Tissue via Mechanical Dissociation using TissueGrinder
Prama Pallavi and Stefan Scheuermann
2. Circulating Tumor Cell Enrichment and Single Cell Isolation Combining the CellSearch® and DEPArray™ Systems
Cäcilia Köstler, Bernhard Polzer, and Barbara Alberter
3. Isolation of Viable Epithelial and Mesenchymal Circulating Tumor Cells from Breast Cancer Patients
Justyna Topa, Anna J. Zaczek, and Aleksandra Markiewicz
4. Single Cell Recovery from Tumor Cell Xenotransplanted Zebrafish Embryos for the Study of Metastasis Initiating Cells
Pablo Hurtado, Inés Martínez-Pena, and Roberto Piñeiro
5. Isolation of Single Circulating Tumor Cells using VyCAP Puncher System
Thais Pereira-Veiga, Bianca Behrens, Joska J. Broekmaat, Lisa Oomens, Michiel Stevens, Arjan G.J. Tibbe, Nikolas Stoecklein, Laura Muinelo-Romay, Roberto Piñeiro, and Clotilde Costa
6. Simultaneous Isolation and Amplification of mRNA and Genomic DNA of a Single Cell
Miodrag Gužvic
7. Isolation and Genomic Analysis of Circulating Tumor Cell Clusters in Cancer Patients
Carolina Reduzzi, Marta Vismara, Thomas Schamberger, Marco Silvestri, Rosita Motta, Bernhard M. Polzer, and Vera Cappelletti
8. Establishing Single Cell Clones from In Vitro Cultured Circulating Tumor Cells
Teng Teng and Min Yu
9. Immunofluorescence Combined with Single-Molecule RNA Fluorescence In Situ Hybridization for Concurrent Detection of Proteins and Transcripts in Stress Granules
Jakub Kochan and Mateusz Wawro
10. Highly Multiplexed and Simultaneous Characterization of Protein and RNA in Single Cells by Flow or Mass Cytometry Platforms using Proximity Ligation Assay for RNA
Andrew D. Duckworth, Joseph R. Slupsky, and Nagesh Kalakonda
11. Array-Based Comparative Genomic Hybridization for the Detection of Copy Number Alterations in Single Cells
Giancarlo Feliciello, Zbigniew Tadeusz Czyz, and Bernhard Polzer
12. Single Cell MicroRNA Sequencing Library Preparation
Sarah M. Hücker and Stefan Kirsch
13. Immunoblot Analysis from Single Cells using Milo™ Single-Cell Western Platform
Prashant V. Thakkar
14. Imaging of Sub-Cellular Distribution of Platinum in Single Cells using Laser Ablation Inductively Coupled Plasma Mass Spectrometry
Amy J. Managh and Calum Greenhalgh
15. Patch-seq: Multimodal Profiling of Single-Cell Morphology, Electrophysiology, and Gene Expression
Cathryn R. Cadwell and Andreas S. Tolias
16. Single-Nucleus ATAC-seq for Mapping Chromatin Accessibility in Individual Cells of Murine Hearts
Michail Yekelchyk, Xiang Li, Stefan Guenther, and Thomas Braun
Subject Index List…
