Selective Autophagy Methods and Protocols

This volume presents the latest techniques used by researchers to study selective autophagy in a broad range of organisms such as yeast, plants, worms, fruit flies, and mammals. The chapters in this book cover several types of selective autophagy such as mitophagy, aggrephagy, ER-phagy, ferritinophagy, pexophagy, and nucleophagy. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Cutting-edge and comprehensive, Selective Autophagy: Methods and Protocols is a valuable resource for novice and expert researchers who want to learn more about this interesting and developing field.

Chapter 3 is available open access under a Creative Commons Attribution 4.0 International License via link.springer.com.

Using Fluorescence Microscopy and Immunoblotting for Monitoring Mitophagy in Budding Yeast.- Methods to Monitor Nucleophagy in Yeast.- Synthetic Dimerization Approaches for In Vivo Studies in Saccharomyces cerevisiae.- Assays to Study Mitophagy in Plants.- Assessing Neuronal Mitophagy during Development in the Nematode Caenorhabditis elegans.- In Vivo Monitoring of Nucleophagy in Caenorhabditis elegans.- Analysis of Mitophagy Reporters in Drosophila.- Studying Selective Autophagy of Protein Aggregates using Particles Induced by Multimerization (PIMs).- Methods for Monitoring ER-phagy.- Quantitative Super-Resolution Imaging of ER-phagy Initiation in Cells.- Fluorescence Methods to Measure Pexophagy.- Methods for Monitoring Mitophagy using mt-Keima.- Monitoring Mitophagy Dynamics in Live Cells.- Methods for Monitoring NCOA4-Mediated Ferritinophagy.- Method to Purify Phase-Separated p62 Bodies using Fluorescence-Activated Particle Sorting.- In Silico Assessment of LIR Motif-Containing Proteins Binding to ATG8-Family Proteins.- High Throughput Screening for LC3/GABARAP Binders Utilizing the Fluorescence Polarization Assay.- Thermodynamic Characterization of LC3/GABARAP:Ligand Interactions by Isothermal Titration Calorimetry.- Determination of hATG8 Binding Selectivity of Autophagy-Interacting Motif (AIM) Peptides Using Native Electrospray Ionization Mass Spectrometry.