RNA Editing Methods and Protocols

This volume provides an overview about main RNA editing mechanisms, focusing on their functions in physiological as well as pathological conditions. Chapters guide readers through state- of-the art methodologies to investigate RNA editing through wet and dry approaches. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and cutting-edge, RNA Editing: Methods and Protocols aims to ensure successful results in the further study of this vital field.

Substitutional RNA Editing in Plant Organelles.- Computational Detection of Plant RNA Editing Events.- Discovering RNA Editing Events in Fungi.- C-to-U RNA Editing: From Computational Detection to Experimental Validation.- Live Cell Quantification of APOBEC1-mediated RNA Editing: A Comparison of RNA Editing Assays.- Adenosine to Inosine RNA Editing Enzyme ADAR and microRNAs.- Quantitative Analysis of Adenosine-to-Inosine RNA Editing.- Discovering A-to-I RNA Editing Through Chemical Methods.- ALU A-to-I RNA Editing - Millions of Sites and Many Open Questions.- RNA Editing in Human and Mouse Tissues.- Bioinformatics Resources for RNA Editing.- High-throughput Sequencing to Detect DNA-RNA Changes.- Detection of A-to-I Hyper-Edited RNA Sequences.- Proteome Diversification by RNA Editing.- MicroRNA Editing Detection and Function: A Combined in silico and Experimental Approach for the Identification and Validation of Putative Oncogenic Targets.- RNA Editing in Interferonopathies.- The Role of RNA Editing in the Immune Response.- RNA Editing in Neurological and Neurodegenerative Disorders.- New Frontiers for Site-directed RNA Editing - Harnessing Endogenous ADARs.