Rhinovirus Methods and Protocols

This volume details methods to detect Rhinovirus (RV) in clinical samples, propagation and purification of virus, in vivo and in vitro model systems, and the newer molecular tools that are useful for the studying the RV infection and pathogenesis.  Chapters guide readers through a greater of understanding of RV pathogenesis in COPD, childhood asthma development and protective innate immunity.  However, there is much to learn about RV infection in order to develop therapies to treat asthma and COPD exacerbations, prevent development of asthma and treat common colds.  Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.

Authoritative and cutting-edge, Rhinovirus: Methods and Protocols aims to be a useful and practical guide to new researchers and experts looking to expand their knowledge.

Detection of virus in clinical samples by PCR.- Laboratory protocol for propagation and purification of rhinovirus A and B suitable for in vitro and in vivo infection.- Method to generate high-titre rhinovirus C for in vitro and in vivo studies.- Quantification of infectious rhinovirus A and B by plaque assay.- Quantifying Rhinovirus Genomic and Negative-Strand RNA by RT-PCR.- Determination of binding and endocytosis of rhinovirus by flow cytometry.- Assessment of viral replication in rhinovirus-infected airway epithelial cells.- Analysis of adherence junctions in rhinovirus-infected airway epithelial cells.- Culturing of airway stem cells obtained from COPD and asthma patients to assess the effects of rhinovirus infection.- Investigation of Differentiated Nasal Epithelial Responses to Infection with Clinical Isolates of Rhinovirus A and C.- In vivo model to assess the nasal infection by minor group rhinovirus.- Identification and function of type 2 innate lymphoid cells in rhinovirus-infected mouse model of asthma.- Assessment of rhinovirus infection in a mouse model of COPD.- Infection of immature mice with rhinovirus for the study of asthma pathogenesis.- In vitro model to test therapeutic agents against rhinovirus.