Recombinant Proteins from Plants Methods and Protocols

This volume provides up-to-date scientific achievements from the world’s top researchers. Recombinant Proteins from Plants: Methods and Protocols, Second Edition guides readers through protocolsfor use with a variety of plant expression systems. Various aspects of production are covered including vector selection and cloning; product improvements for stability, glycosylation, and antibiotic-free selection; extraction and scale-up; and analysis of transgenic plants and their recombinant proteins. Written for the Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and practical, Recombinant Proteins from Plants: Methods and Protocols, Second Edition is an ideal reference for those who are interested in plant molecular biology and molecular farming.

Chloroplast Based Expression of Recombinant Proteins by Gateway® Cloning Technology.- Multigene Engineering in Rice Using High-Capacity Agrobacterium Tumefaciens BIBAC Vectors.- Virus-Derived Vectors for the Expression of Multiple Proteins in Plants.- Transient Protein Expression by Agroinfiltration In Lettuce.- Production of Recombinant Proteins in the Chloroplast of the Green Alga Chlamydomonas Reinhardtii.- Efficient, Antibiotic Marker-Free Transformation of a Dicot and a Monocot Crop with Glutamate 1-Semialdehyde Aminotransferase Selectable Marker Genes.- Transient Expression of Mammalian Genes In Nicotiana Benthamiana To Modulate N-Glycosylation.- Companion Protease Inhibitors for the In Situ Protection of Recombinant Proteins in Plants.- Production of Recombinant Cholera Toxin B Subunit in Nicotiana Benthamiana Using GENEWARE® Tobacco Mosaic Virus Vector.- Total Soluble Protein Extraction for Improved Proteomic Analysis Of Transgenic Plant Roots.- Temporary Immersion Bioreactors for the Contained Production Of Recombinant Proteins In Transplastomic Plants.- Plant Cell-Based Recombinant Antibody Manufacturing with A 200 L Orbitally Shaken Disposable Bioreactor.- Liquid-Liquid Phase Separation of Oil Bodies From Seeds.- Continuous Flow Separation of Hydrophobin Fusion Proteins from Plant Cell Culture Extract.- Molecular Analyses of Transgenic Plants.- Analysis of Recombinant Proteins In Transgenic Rice Seeds: Identity, Localization, Tolerance To Digestion, And Plant Stress Response.- Real-Time PCR-Based Quantitation Method for The Genetically Modified Soybean Line GTS 40-3-2.- A Standardized Lepidopteran Bioassay to Investigate the Bioactivity Of Insecticidal Proteins Produced In Transgenic Crops.- Generating Transgenic Plants Showing Antibody-Mediated Pathogen Resistance.