Recombinant Glycoprotein Production Methods and Protocols

This volume covers a wide spectrum of techniques and approaches that are used in the upstream and downstream processing for recombinant glycoprotein production. Chapters guide the reader through state-of-art of therapeutic recombinant glycoproteins, explores the patent literature, expression systems used for glycoproteins production, methods employed in the downstream processing of different glycoproteins, and information about analytical tools and formulation strategies. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and cutting-edge, Recombinant Glycoprotein Production: Methods and Protocols aims to ensure successful results in the further study of this vital field

Platforms for Recombinant Therapeutic Glycoprotein Production.- Uncovering Innovation Features and Emerging Technologies in Molecular Biology through Patent Analysis.- Production of Full-length Antibody by Pichia Pastoris.- Human cells as Platform to Produce Gamma-Carboxylated Proteins.- Production of Recombinant Factor VIII in Human Cell Lines.- Strategies to Suspension Serum-Free Adaptation of Mammalian Cell Lines for Recombinant Glycoprotein Production.- Production of Recombinant Rabies Virus Glycoprotein by Insect Cells in a Single Use Fixed-bed Bioreactor.- Cell-Free Production of Protein Biologics within 24 hours.- Demonstration-Scale High-Cell-Density Fermentation of Pichia Pastoris.- Large Scale Transient Transfection of Suspension Mammalian Cells for VLP Production.- Bioreactor-Based Production of Glycoproteins in Plant Cell Suspension Cultures.- Fed-Batch CHO Cell Culture for Lab-Scale Antibody Production.- Strategies to Develop Therapeutic N- and O-Hyperglycosylated Proteins.- Expression of Glycosylated Proteins in Bacterial System and Purification by Affinity Chromatography.- Purification Methods for Recombinant Factor VIII Expressed in Human Liver SK-Hep Cells.- Purification Method for Recombinant hG-CSF by Affinity Chromatography.- Microplate-Based Method for High-Throughput Screening (HTS) of Chromatographic Conditions Studies for Recombinant Protein Purification.- Purification and Auto Activation Method for Recombinant Coagulation Factor VII.- Preparation of Immunoliposomes by Direct Coupling of Antibodies Based on a Thioether Bond.- Polyester-Based Nanoparticles for the Encapsulation of Monoclonal Antibodies.- Polyester-Based Nanoparticles for Delivery of Therapeutic Proteins.- Quantification of Coagulation Factor VIII by Selective Reaction Monitoring.