Proteoform Identification Methods and Protocols

Proteoforms and Proteoform Families: Past, Present, and Future.- Membrane Ultrafiltration-Based Sample Preparation Method and Sheath-Flow CZE-MS/MS for Top-Down Proteomics.- Size Exclusion Chromatography Strategies and MASH Explorer for Large Proteoform Characterization.- RPLC-RPLC-MS/MS for Proteoform Identification.- Monolithic Materials-Based RPLC-MS for Proteoform Separation and Identification.- Capillary Isoelectric Focusing: Mass Spectrometry Method for the Separation and Online Characterization of Monoclonal Antibody Charge Variants at Intact and Subunit Level.- Proteoform Analysis and Construction of Proteoform Families in Proteoform Suite.- Top-Down Mass Spectrometry Data Analysis Using TopPIC Suite.- Accurate Proteoform Identification and Quantitation Using pTop 2.0.- Proteoform Identification and Quantification using Intact Protein Database Search Engine ProteinGoggle.- Mass Deconvolution of Top-Down Mass Spectrometry Datasets by FLASHDeconv.- Deconvolving Native and Intact Protein Mass Spectra with UniDec.- Discovery of Unknown Post-Translational Modifications by Top-Down Mass Spectrometry.- Determining Copper and Zinc Content in Superoxide Dismutase using Electron Capture Dissociation under Native Spray Conditions.- Surface-Induced Dissociation for Protein Complex Characterization.