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morgan david m. l. (curatore) - polyamine protocols

Polyamine Protocols




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Dettagli

Genere:Libro
Lingua: Inglese
Editore:

Humana Press

Edizione: 1998





Trama

David Morgan brings together in Polyamine Protocols a unique collection of hands-on enzyme assay techniques for the study of polyamines and their biological functions. The techniques range from assay methods for enzymes of polyamine biosynthesis and catabolism to measurements of polyamines, polyamine transport, and polyamine effects on cell growth. All methods are presented by active researchers who have developed them to a high degree of perfection in their own laboratories, and include clear, step-by-step instructions with numerous hints and tips to ensure readily reproducible results.

Polyamine Protocols is the only compilation of methods currently available for use in studies involving polyamines. The collection offers both novice and experienced researchers across many fields-including cell and molecular biology, pharmacology, oncology, physiology, laboratory medicine, and clinical pathology-all the tools needed to elucidate the biological roles played by these ubiquitous substances.




Sommario

Polyamines: An Introduction.- Assay Methods for Enzymes of Polyamine Biosynthesis.- Determination of Ornithine Decarboxylase Activity Using [3H]Omithine.- Assay of Mammalian Ornithine Decarboxylase Activity Using [14C]Ornithine.- Assay of Mammalian S-Adenosylmethionine Decarboxylase Activity.- Assay of Spermidine and Spermine Synthases.- Measurement of Spermidine/Spermine N 1-Acetyltransferase Activity.- Assay of Spermidine N 8-Acetyltransferase.- Assay Methods for Enzymes of Polyamine Catabolism.- Assay of N 8-Acetylspermidine Deacetylase.- Hydrogen Peroxide Assay for Amine Oxidase Activity.- Radiochemical Assay of Diamine Oxidase.- Radiochemical Estimation of Polyamine Oxidase.- Measurement of Aldehyde Formation as an Assay for Polyamine Oxidase Activity.- Measurement of Polyamines.- Determination of Polyamines as Their Benzoylated Derivatives by HPLC.- A Dansyl Chloride-HPLC Method for the Determination of Polyamines.- The Determination of Polyamines and Amino Acids by a Fluorescamine-HPLC Method.- Thin-Layer Chromatographic Method for Assaying Polyamines.- Measurement of Polyamine Transport.- Measurement of Polyamine Transport: Adherent Cells.- Measurement of Polyamine Transport: Cells in Suspension.- Measurement of Polyamine Efflux from Cells in Culture.- Measurement of Polyamine Effects on Cell Growth.- Leucine Incorporation and Thymidine Incorporation.- Tetrazolium (MTT) Assay for Cellular Viability and Activity.










Altre Informazioni

ISBN:

9780896034488

Condizione: Nuovo
Collana: Methods in Molecular Biology
Dimensioni: 233 x 155 mm
Formato: Copertina rigida
Illustration Notes:IX, 186 p.
Pagine Arabe: 186
Pagine Romane: ix


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