Plant Stress Tolerance Methods and Protocols

This fully updated new edition explores new techniques for studying plant stress. This includes novel methodologies such as MeRIP-seq for identifying changes in m6A profiles, isolation of stress granules, and additional methodologies such as MNase–seq for identifying nucleosome occupancy, alternative splicing analysis, identifying proteins that interact with long noncoding RNAs, untargeted metabolomics, ROS and NO measurements, priming-related protocols, growth-promoting bacteria isolation and functional characterization, as well as isolating mutants for stress-regulated genes using CRISPR technology. Written for the highly successful Methods in Molecular Biology series, chapters feature introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and up-to-date, Plant Stress Tolerance: Methods and Protocols, Third Edition provides a wide range of protocols catering to the needs of plant physiologists, biochemists, and molecular biologists interested in probing this vital area of study.

Strategies of Molecular Signal Integration for Optimized Plant Acclimation to Stress Combinations.- MNase-Seq Analysis for Identifying Stress-Altered Nucleosome Occupancy in Plants.- MeRIP-Seq for Identifying Stress-Responsive Transcriptome-Wide m6A Profiles in Plants.- Isolation and Visualization of Plant Stress Granule-Associated Components via On-Beads Digestion and Co-Localization Analysis.- Determination of Differential Alternative Splicing Under Stress Conditions.- A Method to Quantitatively Examine Heat Stress-Induced Alternative Splicing in Plants by RNA-Seq and RT-PCR.- Thiol Redox Proteomics for Identifying Redox-Sensitive Cysteine Residues Within the Protein of Interest During Stress.- Detection of Stress-Induced Changes in Subcellular Protein-Distribution.- Validation of the Interaction Between Stress-Responsive LncRNAs and RNA-Binding Proteins In Vitro.- Measurement of Photorespiratory Cycle Enzyme Activities in Leaves Exposed to Abiotic Stress.- In Vitro Kinase Assay with Recombinant SnRK2s: An Example for Assaying Stress-Responsive Kinases in Plants.- Identification of Stress-Responsive Metabolites in Plants Using an Untargeted Metabolomics Approach.- Measurement of Reactive Oxygen Species and Nitric Oxide from Tomato Plants in Response to Abiotic and Biotic Stresses.- Reactive Oxygen Species (ROS) Measurement in Arabidopsis Guard Cells in Response to Biotic and Abiotic Stresses.- Detection of Reactive Oxygen Species in Plant Root Immunity.- Studying Thermopriming-Mediated Short- and Long-Term Acquired Thermotolerance in Arabidopsis thaliana.- X-Ray Film Contact Print Method for Analyzing Differential Expression of Plant Protease Inhibitors Under Abiotic Stress.- Isolation and Identification of Growth-Promoting Bacteria from Plants Growing Under Abiotic Stresses.- Functional Characterization and Localization of Plant-Growth Promoting Bacteria Grown Under Stressful Conditions.- Generating Mutant for a Stress-Responsive Gene in Rice Using CRISPR-Cas9 System.