Photosynthesis Methods and Protocols

This fully updated edition explores fundamental protocols for the study of photosynthesis in a manner accessible to a broad spectrum of researchers. Featuring protocols to examine light response curves and gas exchange measurements for a variety of samples, the book includes new protocols on photosynthesis in the adaxial and abaxial sides of a leaf, non-foliar organs, and aquatic systems. Protocols and best practice for eddy covariance, thermal, spectral, and hyperspectral imaging and data analysis, as well as stable isotope labeling and quantification of photosynthetic metabolites, are introduced alongside fresh insights on many more topics. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Tools for Measuring Photosynthesis at Different Scales.- Light Response Curves in Land Plants.- Photosynthetic Gas Exchange in Land Plants at the Leaf Level.- Simultaneous and Independent Abaxial and Adaxial Gas Exchange Measurements.- Measuring Non-Foliar Photosynthesis.- Measurements of Carbon Assimilation in Aquatic Systems.- Measurement of Algal Photosynthesis Using a Clark-Type O₂ Electrode.- Measurements of Oxygen Evolution in Photosynthesis.- Measurement of O₂ Uptake and Evolution in Leaves In Vivo Using Stable Isotopes and Membrane Inlet Mass Spectrometry.- Using Carbon Stable Isotopes to Study C₃ and C₄ Photosynthesis: Models and Calculations.- Measuring Canopy Gas Exchange Using CAnopy Photosynthesis and Transpiration Systems (CAPTS).- A Beginner’s Guide to Eddy Covariance: Methodology and Its Applications to Photosynthesis.- Chlorophyll Fluorescence on the Fast Timescale.- Shining Light into a ‘Black Box’: Essential RationaleUnderlying Multiphase Flash Methodology.- Imaging Spatial and Temporal Variation in Photosynthesis Using Chlorophyll Fluorescence.- Using Infrared Thermography for High Throughput Plant Phenotyping.- Spectral Reflectance Measurements.- Hyperspectral Proximal Sensing for Estimating Photosynthetic Capacities at Leaf and Canopy Scales.- Remote Sensing Techniques: Hyperspectral Imaging and Data Analysis.- Extraction of Soluble Proteins from Leaves.- Antibody Design for the Quantification of Photosynthetic Proteins and Their Isoforms.- Purification of Rubisco from Leaves.- Evaluation of Lipids for the Study of Photosynthetic Membranes.- Stable Isotope Labeling and Quantification of Photosynthetic Metabolites.