Phage Display Methods and Protocols

This volume provides comprehensive explanations and detailed examples of different antibody libraries, along with novel approaches for antibody discovery. The chapters in this book are divided into four sections: 1) construction of antibody libraries; 2) selection strategies for antibodies; 3) complementary approaches for antibody selection; and 4) phage display for epitope mapping and biomarker identification. The chapters also provide a list of antibody phage display technologies and applications. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Cutting-edge and practical, Phage Display: Methods and Protocols will provide technical assistance to new start-ups venturing into the field of antibody phage display.

This volume will also aid in stirring interest and ideas among researchers in this ever-expanding subject.

play Approach.- Parallelized Antibody Selection in Microtiter Plates.- Mass Spectrometry Immuno Assay (MSIA ™) Streptavidin Disposable Automation Research Tips (D.A.R.T’s®) Antibody Phage Display Biopanning.- Magnetic Nanoparticle-Based Semi-Automated Panning for High-Throughput Antibody Selection.- Phage Display and Selections on Cells.- Combine Phage Antibody Display Library Selection on Patient Tissue Specimens with Laser Capture Microdissection to Identify Novel Human Antibodies Targeting Clinically Relevant Tumor Antigens.- Antibody Isolation Form a Human Synthetic Combinational and Other Libraries of Single-Chain Antibodies.- Screening Phage Display Antibody Libraries using Protein Arrays.- Antibody Selection on FFPE Tissue Slides.- Antibody Affinity and Stability Maturation by Error Prone PCR.- Upgrading Affinity Screening Experiments by Analysis of Next Generation Sequencing Data.- Next-Generation DNA Sequencing of VH/VL Repertoires: A Primer and Guide to Applications in Single-Domain Antibody Library Construction.- High-Throughput IgG Reformatting and Expressions.- Monitoring Phage Biopanning by Next-Generation Sequencing.- ORFeome Phage Display.- Epitope Mapping by Phage Display.- Metasecretome Phage Display.- Phagekines: Screening Binding Properties and Biological Activity of Functional Cytokines Displayed on Phages.