Peptide Nucleic Acids Methods and Protocols

Exemplifying and illustrating recent exciting advances in PNA chemistry, the second edition of Peptide Nucleic Acids: Methods and Protocols serves as a vital complement to the first edition of the book. Since the discovery of peptide nucleic acids, many interesting new derivatives and analogues in terms of nucleic acid recognition specificity and affinity have emerged. Also, as this detailed volume presents, great ingenuity in exploiting the unique properties of PNAs for a wide variety of applications within drug discovery, medical diagnostics, chemical biology and nanotechnology has unfolded. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.

Peptide Nucleic Acids: Methods and Protocols, Second Edition serves as a source of useful specific methods and protocols as well as a source of inspiration for future developments.

MiniPEG-gPNA.- Cyclopentane Peptide Nucleic Acids.- Chiral PNAs with Constrained Open-chain Backbones.- Synthesis, Characterization and Evaluation of Radiometal-Containing Peptide Nucleic Acids.- Preparation of Metal-Containing Peptide Nucleic Acid Bioconjugates on the Solid Phase.- Formation and Characterization of PNA-Containing Heteroquadruplexes.- Sequence Selective Recognition of Double Stranded RNA using Triple Helix-forming Peptide Nucleic Acids.- Assembly of PNA-tagged Small Molecules, Peptides and Carbohydrates onto DNA Templates: Programming the  Combinatorial Pairing and Inter-ligand Distance.- Site-selective Scission of Human Genome using PNA-based Artificial Restriction DNA Cutter.- PNA Openers and their Applications for Bacterial DNA Diagnostics.- DNA-templated Native Chemical Ligation of Functionalized Peptide Nucleic Acids – A Versatile Tool for Single Base Specific Detection of Nucleic Acids.- Use of Peptide Nucleic Acids (PNAs) for Genotyping by Solution and Surface Methods.- Analysis of PNA Hybridization by Surface Plasmon Resonance.- PNA Fluorescent in situ Hybridization (FISH) For Rapid Microbiology and Cytogenetic Analysis.- Rapid miRNA Imaging in Cells using Fluorogenic Templated Staudinger Reaction Between PNA-based Probes.- Cellular Delivery of Peptide Nucleic Acids (PNAs).- Peptide Nucleic Acid Mediated Recombination For Targeted Genomic Repair and Modification.- Antisense Effects of PNAs in Bacteria.