Patient-Derived Xenografts Methods and Protocols

This detailed volume explores the dynamic landscape of patient-derived xenograft (PDX) generation and applications. After a few chapters examining the evolution of PDXs, tracing their roots and contextualizing their significance in the broader narrative of cancer research, the book continues with methodologies for generating PDXs from diverse clinical tissue samples as well as for utilizing PDXs in various specific cancer types. From the development of humanized PDX models to the exploration of protein expression through advanced techniques including multiplex immunohistochemistry, Western blotting, and immunoprecipitation, the chapters offer a comprehensive toolkit for researchers and clinicians alike. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Patient-Derived Xenografts: Historical Evolution, Immunocompromised Host Models, and Translational Significance.- Patient-Derived Xenograft Models in Cancer Research: Methodology, Applications, and Future Prospects.- Patient-Derived Xenografts: A Valuable Preclinical Model for Drug Development and Biomarker Discovery.- Patient-Derived Xenograft Models for Leukemias.- Patient-Derived Orthotopic Xenograft Models for High-Grade Pediatric Brain Cancers.- Generation of Orthotopic and Subcutaneous Patient-Derived Xenograft Models from Diverse Clinical Tissue Samples of Pediatric Extracranial Solid Tumors.- Development of Orthotopic Patient-Derived Xenograft Models of Pediatric Intracranial Tumors.- A Humanized Patient-Derived Xenograft Model for Pancreatic Cancer.- Development and Expansion of Patient-Derived Xenografts for Endometrial Cancer.- A Multiplexed Approach to Assess Small Cell Lung Cancer Subtype Heterogeneity in Primary and Patient-Derived Tumor Samples.- Generation of Metastatic Cholangiocarcinoma Patient-Derived Xenograft Models.- Patient-Derived Xenograft Models for Translational Prostate Cancer Research and Drug Development.- Patient-Derived Xenograft Models for Ovarian Cancer.- Orthotopic MC-38 Allograft as a Robust Preclinical Model of Colorectal Carcinoma.- Assessing Protein Expression in Patient-Derived Xenografts Using Western Blotting.- Analysis of Protein Interactions in Patient-Derived Xenografts Using Immunoprecipitation.- A Semi-Automated Proteomics and Phosphoproteomics Protocol for the Identification of Novel Therapeutic Targets and Predictive Biomarkers in In Vivo Xenograft Models of Pediatric Cancers.