NMDA Receptor Protocols

Min Li and a panel of hands-on experimentalists detail state-of-the-art molecular techniques for studying NMDA ligand-gated ion channels and developing assays for nontherapeutic lead selection. The topics range from cDNA cloning to in vitro and in vivo investigation of the channel complex in the mammalian brain. Additional topics include the biochemical analysis of the channel protein and the construction of various heterologous systems for both basic research and high throughput screens (HTS) for pharmaceutical chemicals. Although the focus is on NMDA receptors, the methods are applicable to other ligand-gated ion channels and with some modification may be extended to related membrane signaling receptors. NMDA Receptor Protocols offers today's scientists powerful methods for basic research on NMDA receptor structure and function, as well as enormous opportunities for clinical investigation toward the development of novel bioactive compounds.

Isolation of Receptor Clones by Expression Screening in Xenopus Oocytes, Fumio Nakamura, Yoshio Goshima, Stephem M. Struttmatter, and Susumu Kawamoto. Expression of NMDA Receptor Channel Subunit Proteins using Baculovirus and Herpesvirus Vectors, Susumu Kawamoto, Satoshi Hattori, Shigeo Uchino Masayoshi Mishina, and Kenji Okuda. Transient Expression of Functional NMDA Receptors in Mammalian Cells, Paul L. Chazot, Miroslav Cik, and F. Anne Stephenson. Stable Expression of Human NMDA Receptors in Cultured Mammalian Cells, Mark A. Varney, Fen-Fen Lin, Christine Jachec, Sara P. Rao, Stephen D. Hess, Edwin C. Johnson, and Gönül Veliçelibi. Expression of Extracellular N-terminal Domain of NMDA Receptor in Mammalian Cells, Erik A. Whitehorn, William J. Dower, and Min Li. Immunocytochemistry of NMDA Receptors, Ronald S. Petralia and Robert J. Wenthold. The Use of Ligand Binding in Assays of NMDA Receptor Function, Ian J. Reynolds and Terre A. Sharma. Calmodulin Modification of NMDA Receptors, Su Zhang and Richard L. Huganir. Detergent Solubilization and Immunoprecipitation of Native NMDA Receptors, Robert J. Wenthold, Jaroslav Blahos II, Kyung-Hye Huh, and Ronald S. Petralia. Redox Sensitivity of NMDA Receptors, Stuart Lipton. In Vitro Selection of Peptides Acting on NMDA Receptors, Steven E. Cwirla, William Dower, and Min Li. NMDA Receptor Pharmacololgy and Analysis of Patch-Clamp Receptors, Jerry M. Wright and Robert W. Peoples. Determination of Membrane Topology of Glutamate Receptors, Hendrika M.A. VanDongen and Antonius M.J. VanDongen. Engineering the NMDA Receptor Channel Lining, Antonio V. Ferrer-Montiel and Mauricio Montal. High-Throughput Functional Detection of NMDA Receptor Activity, Martin Donal Dunningham and Sara Mary Cunningham. Index.