Neuroprotection Method and Protocols

This volume contains cutting-edge molecular biology methods on neuroprotective mechanisms and specific preclinical models of the CNS injury, iseases and planning translation. Chapters guide readers through neuropathology, neuroprotection, Alzheimer’s disease, amyotrophic lateral sclerosis, ALS, Huntington’s disease , multiple sclerosis, Parkinson’s disease, spinal cord injury, traumatic brain injury, and ischemic brain injury. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

 Authoritative and cutting-edge, Neuroprotection: Methods and Protocols aims to ensure successful results in the further study of this vital field.

 

TUNEL-n-DIFL Method for Detection and Estimation of Apoptosis Specifically in Neurons and Glial Cells in Mixed Culture and Animal Models of Central Nervous System Diseases and Injuries.- Isolation of Capillaries from Small Amounts of Mouse Brain Tissue.- Isolation of Extracellular Vesicles Using Formulas to Adapt Centrifugation to Different Centrifuges.- High-resolution Respirometry for Mitochondrial Function in Rodent Brain.- Quantification of Neuronal Dendritic Spine Density and Lengths of Apical and Basal Dendrites in Temporal Lobe Structures Using Golgi-Cox Staining.- Quantification of Neuroinflammatory Markers in Blood, Cerebrospinal Fluid, and Resected Brain Samples Obtained from Patients.- Targeted Modification of Epigenetic Marks Using CRISPR/Dcas9-Suntag-based Modular Epigenetic Toolkit.- Elevated Plus Maze for Assessment of Anxiety and Memory in Rodents.- Drosophila Neuromuscular Junction as a Model to Study Synaptopathies and Neuronal Autophagy.- Cell-based Assay to Detect the Autoantibody Serostatus in Patients with Neuromyelitis Optica Spectrum Disorder (NMOSD).- Using Small Molecules for Targeting Heavy Metals in Neurotoxicity and Neuroinflammation.- Chromatographic Separation and Quantitation of Sphingolipids from the Central Nervous System or Any Other Biological Tissue.- Role of Network Pharmacology in Prediction of Mechanism of Neuroprotective Compounds.- Role of Serotonergic System in Regulating Brain Tumor-associated Neuroinflammatory Responses.- Impacts of Omega-3 Fatty Acids, Natural Elixirs for Neuronal Health, on Brain Development and Functions.- Microglial Uptake of Extracellular Tau by Actin-mediated Phagocytosis.- Internalization and Endosomal Trafficking of Extracellular Tau in Microglia Improved by a-Linolenic Acid.- Understanding Actin Remodeling in Neuronal Cells Through Podosomes.- Quantitative Investigation of Neuroprotective Role of ROR1in a Cell Culture Model of Alzheimer's Disease.- microRNA Isolation, Expression Profiling, and Target Identification for Neuroprotection in Alzheimer's Disease.- Quantitative Measurement of Tau Aggregation in Genetically Modified Rats with Neurodegeneration.- Detection and Characterization of Apoptosis-related Proteins in Hippocampal Neurodegeneration: From mRNA Expression to Protein Quantification.- Isolation and Detection of Pathological Tau Species in a Tauopathy Mouse Model.- Enzyme Inhibition Assays for Monoamine Oxidase.- Role of Amyloid Beta in Neurodegeneration and Therapeutic Strategies for Neuroprotection.- Amyloid Beta Mediated Neurovascular Toxicity in Alzheimer’s Disease.- Fecal Microbiota Transplantation in Amyotrophic Lateral Sclerosis: Clinical Protocol and Evaluation of Microbiota Immunity Axis.- Integrated Multi-omics Analysis and Validation in Yeast Model of Amyotrophic Lateral Sclerosis.- Imaging and Assay of the Dynamics of Cytotoxic Huntingtin (HTT) Protein Aggregates Regulated by lncRNAs.- Astrocyte Activation and Drug Target in Pathophysiology of Multiple Sclerosis.- Promises of Lipid-based Nanocarriers for Delivery of Dimethyl Fumarate to Multiple Sclerosis Brain.- Chrysin for Neurotrophic and Neurotransmitter Balance in PD.- Establishment of a 6-OHDA Unilateral Lesion Male Wistar Rat Model of Parkinson's Disease.- Evaluating Motor Dysfunction and Oxidative Stress Induced by Trichloroethylene in Wistar Rats.- Neurobehavioral Analysis to Assess Olfactory and Motor Dysfunction in Parkinson’s Disease.- Ion Channels and Metal Ions in Parkinson’s Disease: Historical Perspective to the Current Scenario.- Creating a Reproducible Model of Spinal Cord Injury in Rats: A Contusion Approach.- Weight Drop Method for Inducing Closed Head Diffuse Traumatic Brain Injury.- iDISCO Tissue Clearing Whole-Brain and Light Sheet Microscopy for High-ThroughputImaging in a Mouse Model of Traumatic Brain Injury.- Insights From Rodent Models for Improving Bench-to-Bedside Translation in Traumatic Brain Injury.- Rat Model of Middle Cerebral Artery Occlusion.