Neurobiology Methods and Protocols

This volume will serve as a guide for students in the field of neurobiology, and be a bridge between basic science researchers, doctors, and surgeons in clinical practice. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and cutting-edge, Neurobiology: Methods and Protocols aims to ensure successful results in the further study of this vital field.

Plate-based assays for the characterisation of mitochondrial and cellular phenotypes.- Behavioural tests for associative learning in Caenorhabditis elegans.- Mechanical ablation of larval zebrafish spinal cord.- mHigh-throughput multiplex immunohistochemistry of glioma organoids.- The bilateral carotid artery stenosis (BCAS) model of vascular dementia.- In-situ hybridisation to characterise neurulation and midbrain-hindbrain boundary formation in zebrafish.- Administering a behavioural test battery in rodents.- A simple method for quantifying larval locomotion in Drosophila melanogaster.- Neural Stem/Progenitor Cell (NSPC) extraction and culture.- Testing prepulse inhibition of acoustic startle in rodents.- Syngeneic mouse model of glioblastoma: intracranial implantation of GL261 cells.- Measurement of murine neuromuscular function using the in-situ preparation.- Cryosectioning and immunohistochemistry using frozen adult murine brain neural tissue.- Phenotypic analysis of early neurogenesis in a mouse chimeric embryo and stem-cell based neuruloid model.- Active induction of a multiple sclerosis-like disease in common laboratory mouse strains.-Immunohistochemical analysis of the Drosophila Larval Neuromuscular Junction.- Neural endophenotype assessment in zebrafish larvae using optomotor and zebrabox locomotion assessment.-The Photothrombotic Model of Ischaemic Stroke.