Neural Stem Cells Methods and Protocols

This detailed volume explores techniques for the study of neurogenic niches and neural lineage progression, specifically addressing major methodological challenges in studying neural stem cells (NSCs) and related cell populations, such as astrocytes, oligodendrocytes, and neurons. The chapters are organized to offer a comprehensive technical foundation, focusing on isolation and identification, monitoring, manipulation, analysis, and the exploration of potential therapeutic applications of NSCs. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and practical, Neural Stem Cells: Methods and Protocols serves as an ideal guide for the scientific community and a broader audience interested in understanding neurogenic niches and the progression of neural lineages.

Flow-Cytometry-Based Protocols for the Mouse Subependymal Neurogenic Niche Phenotyping.- Isolation of Adult Hippocampal Neural Stem Cells.- Low Density Primary Cell Culture of Postnatal Murine Cerebellar Progenitors In Vitro.- Isolation of Oligodendrocyte Precursor Cells with Immunomagnetic Beads for Their Better Viability In Vitro and In Vivo.- Non-Hematopoietic Stem Cell Identification and Sorting in Adult Retina.- Neurospheres from Ovarian Cortical Cells in Mammals: Generation In Vitro and Neurosphere Assay.- Detection of Monocytic Myeloid-Derived Suppressor Cells in the Human Brain and Blood.- Cell-Type Specific Protein Metabolic Labeling and Identification Using the Methionine Subrogate ANL in Cells Expressing a Mutant Methionyl-tRNA Synthetase.- Experimental Neurogenesis in the Embryos of the Gecko Paroedura picta.- Genetic Tracing of Progenitors from Embryo to Postnatal Brain.- Tracking Neural Migration in Dentate Gyrus Using Thymidine Analogous.- Electroporation Techniques to Target Subependymal Zone Neurogenic Niche.- In Vitro and In Vivo Direct Reprogramming of Astrocytes to Induced-Neurons.- Retrovirus-Mediated Reprogramming of Endogenous Hippocampal Glia into GABAergic Induced Neurons.- Gene Expression Manipulation via Acute Electroporation in Human Brain Organoids.- Targeted Human Editing Using CRISPR Technology in Neural Stem Cells.- Patch-Clamp Recordings in Isolated Neural Cells, Acute Tissue Slices, and Brain Rosettes.- Acute Slice Patch-Clamp Recordings to Characterize Neural Populations in the Hippocampal Subgranular Zone.- Intracellular Calcium Recording and Immunostaining of Single Neural Populations In Vitro.- Cell Population Analyses of Neurospheres Generated In Vitro from Ovarian Cortical Cells in Mammals: Characterization by Immunohistochemistry and Flow Cytometry.- Reference-Based Gene Expression Analysis Using Galaxy Server.- Development of Novel Neuroprotective Agents Targeting GPCR Using In Vitro Assays.- Picrotoxin-Induced Epileptic Hippocampal Organotypic Slice Cultures (hOTCs).