Natural Product Isolation and Identification Methods and Protocols

The purpose of this book is to provide guidance in the process of extraction, separation, and identification of natural products. Chapters guide readers through HPLC, GC, NMR, TLC, deep eutectic solvents, chemical elucidation of individual groups of metabolites,  phenolics, coumarins, lignans, saponins, and their integration with a biological model. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and cutting-edge, Natural Product Isolation and Identification: Methods and Protocols aims to be a reference guide for natural products researchers or researchers beginning practical work in the natural products field.

Natural products analysis through time: from past achievements to future prospect.- Optimizing extraction solvents for plant chemical analysis using 1H NMR and principal component analysis.- High-resolution isolation of natural products: efficient combination of dryload injection and HPLC gradient transfer.- Standardized HPTLC for reproducible Chemical Fingerprinting.- Extraction of anthocyanins with deep eutectic solvents and chemical evaluation by high performance liquid chromatography.- Extraction of essential oils and terpene volatiles from plants and identification by GC-MS based techniques.- Isolation of natural products from marine invertebrates.- Identification and profiling of primary metabolites through GC-MS and associated data processing.- Identification of flavonoids using UV-VIS and MS spectra.- Analysis of salicylate and phenolic acids in plant by HPLC-fluorescence detector.- Isolation and identification of coumarins from Citrus latifolia leaves using advanced chromatographic techniques.- Identification of Lignans.- Extraction, annotation, and purification of resin glycosides from the morning glory family (Convolvulaceae).- Extraction, isolation and structure elucidation of acylated triterpene saponins from Asteraceae family.- Analysis and identification of polyphenols in Fragaria, Rubus and Ribes species by non-targeted UHPLC-PDA-MS metabolite profiling.- Identification of primary metabolites in 1H NMR-based metabolomics of plants.- Isolation of natural products using preparative-TLC.- Sample preparation of Caenorhabditis elegans for GC-MS based metabolomics in toxicity assessment.