Part I: Reviews
1. Application of Multiplex Biomarker Approaches to Accelerate Drug Discovery and Development
Hassan Rahmoune and Paul C. Guest
2. The Application of Multiplex Biomarker Techniques for Improved Stratification and Treatment of Schizophrenia Patients
Johann Steiner, Paul C. Guest, Hassan Rahmoune, and Daniel Martins-de-Souza
3. Multiplex Biomarker Approaches in Type 2 Diabetes Mellitus Research
Susan E. Ozanne, Hassan Rahmoune, Daniel Martins-de Souza, and Paul C. Guest
4. LC-MSE, Multiplex MS/MS, Ion Mobility, and Label-Free Quantitation in Clinical Proteomics
Gustavo Henrique Martins Ferreira Souza, Paul C. Guest, and Daniel Martins-de-Souza
5. Phenotyping Multiple Subsets of Immune Cells In Situ in FFPE Tissue Sections: An Overview of Methodologies
James R. Mansfield
Part II: Statistical Considerations
6. Identification and Clinical Translation of Biomarker Signatures: Statistical Considerations
Emanuel Schwarz
7. Opportunities and Challenges of Multiplex Assays: A Machine Learning Perspective
Junfang Chen and Emanuel Schwarz
Part III: Protocols
8. Multiplex Analyses Using Real-Time Quantitative PCR
Steve F.C. Hawkins and Paul C. Guest
9. Multiplex Analysis Using cDNA Transcriptomic Profiling
Steve F.C. Hawkins and Paul C. Guest
10. Multiplex Single Nucleotide Polymorphism Analyses
Steve F.C. Hawkins and Paul C. Guest
11. Pulsed SILAC as a Proteomic Approach for miRNA Targets Identification in Cell Culture
Daniella E. Duque-Guimarães, Juliana de Almeida-Faria, Thomas Prates Ong, and Susan E. Ozanne
12. Blood Bio-Sampling Procedures for Multiplex Biomarkers Studies
Paul C. Guest and Hassan Rahmoune
13. Multiplex Immunoassay Profiling
Laurie Stephen
14. Multiplex Sequential Immunopreciptation of Insulin Secretory Granule Proteins from Radiolabeled Pancreatic Islets
Paul C. Guest
15. Two Dimensional Gel Electrophoresis of Insulin Secretory Granule Proteins from Biosynthetically-Labeled Pancreatic Islets
Paul C. Guest
16. Depletion of Highly Abundant Proteins of the Human Blood Plasma: Applications in Proteomics Studies of Psychiatric Disorders
Sheila Garcia, Paulo A. Baldasso, Paul C. Guest, and Daniel Martins-de-Souza
17. Simultaneous Two-Dimensional Difference Gel Electrophoresis (2D-DIGE) Analysis of Three Distinct Proteomes
Adriano Aquino, Paul C. Guest, and Daniel Martins-de Souza
18. Selective Reaction Monitoring for Quantitation of Cellular Proteins
Vitor M. Faça
19. Characterization of a Protein Interactome by Co-Immunoprecipitation and Shotgun Mass Spectrometry
Giuseppina Maccarrone, Juan Jose Bonfiglio, Susana Silberstein, Christoph W. Turck, and Daniel Martins-de-Souza
20. Using 15N-Metabolic Labeling for Quantitative Proteomic Analyses
Maccarrone Giuseppina, Alon Chen, and Michaela D. Filiou
21. Multiplex Measurement of Serum Folate Vitamers by UPLC-MS/MS
Sarah Meadows
22. UPLC-MS/MS Determination of Deuterated 25-Hydroxyvitamin D (d3-25OHD3) and Other Vitamin D Metabolites for the Measurement of 25OHD Half-Life
Shima Assar, Inez Schoenmakers, Albert Koulman, Ann Prentice, and Kerry S. Jones
23. iTRAQ-Based Shotgun Proteomics Approach for Relative Protein Quantification
Erika Velásquez Núñez, Gilberto Barbosa Domont, and Fábio César Sousa Nogueira
24. 1H NMR Metabolomic Profiling of Human and Animal Blood Serum Samples
João G.M. Pontes, Antonio J.M. Brasil, Guilherme C.F. Cruz, Rafael N. de Souza, and Ljubica Tasic
25. Lab-on-a-Chip Multiplex Assays
Harald Peter, Julia Wienke, and Frank F. Bier
26. Multiplex Smartphone Diagnostics
Juan L. Martinez-Hurtado, Ali K. Yetisen, and Seok H. Yun
27. Development of a User-Friendly App for Assisting Anticoagulation Treatment
Johannes Vegt
Part IV: Future Directions
28. Multiplex Biomarker Approaches to Enable Point-of-Care Testing and Personalized Medicine
Paul C. Guest
