Part I: Reviews
1. Multiplex Technologies in COVID-19 Research, Diagnostics, and Prognostics: Battling the Pandemic
Paul C. Guest, Fatemeh Zahedipour, Muhammed Majeed, Tannaz Jamialahmadi, and Amirhossein Sahebkar
2. Multivalent Vaccine Strategies in Battling the Emergence of COVID-19 Variants
Paul C. Guest
3. Challenges of Multiplex Assays for COVID-19 Research: A Machine Learning Perspective
Paul C. Guest, David Popovic, and Johann Steiner
Part II: Protocols
4. Multiplex Quantitative Polymerase Chain Reaction Diagnostic Test for SARS-CoV-2 and Influenza A/B Viruses
Steve F.C. Hawkins and Paul C. Guest
5. Multiplex Quantitative Polymerase Chain Reaction Test to Identify SARS-CoV-2 Variants
Steve F.C. Hawkins and Paul C. Guest
6. NIRVANA for Simultaneous Detection and Mutation Surveillance of SARS-CoV-2 and Co-Infections of Multiple Respiratory Viruses
Chongwei Bi, Gerardo Ramos-Mandujano, and Mo Li
7. Quantitative Real-Time RT-PCR Systems to Detect SARS-CoV-2
Sumino Yanase, Hiroyoshi Sasahara, Momoko Nabetani, Kensuke Yamazawa, Keisuke Aoyagi, Akiko Mita, Yuichi Honma, and Yasuhiko Chiba
8. Guidance for SARS-CoV-2 RNA-Based Molecular Assay Analytical Performance Evaluations
Lara Noble, Lesley Scott, Riffat Munir, Kim Steegen, Mignon du Plessis, Lucia Hans, and Wendy Stevens
9. A Rapid User-Friendly Lab-on-a-Chip Microarray Platform for Detection of SARS-CoV-2 Variants
Emily Mattig, Paul C. Guest, and Harald Peter
10. SARS-CoV-2 Host Immunogenetic Biomarkers
Maemu P. Gededzha, Nakampe Mampeule, Anastasia Gandini, and Elizabeth S. Mayne
11. FnCas9 Editor Linked Uniform Detection Assay for COVID-19
Rhythm Phutela, Sneha Gulati, Manoj Kumar, Souvik Maiti, and Debojyoti Chakraborty
12. Mass Spectrometry Multiplexed Detection of SARS-CoV-2
Luciana Godoy Viana, Adriana Lebkuchen, Rodrigo Andrade Schuch, Guilherme Gonçalves Okai, Jessica Silva Salgueiro, Karina Helena Morais Cardozo, and Valdemir Melechco Carvalho
13. Identification of Circulating Biomarkers of COVID-19 Using MALDI-TOF Mass Spectrometry
Lucas C. Lázari, Livia Rosa-Fernandes, and Giuseppe Palmisano
14. Antibody-Based Affinity Capture Combined with LC-MS Analysis for Identification of COVID-19 Disease Serum Biomarkers
Paul C. Guest and Hassan Rahmoune
15. Liquid Chromatography Mass Spectrometry Analysis of Peripheral Blood Mononuclear Cells from SARS-CoV-2 Infected Patients
Paul C. Guest and Hassan Rahmoune
16. Assay of Fatty Acids and Their Role in the Prevention and Treatment of COVID-19
Tharusha Jayasena, Sonia Bustamante, Anne Poljak, and Perminder Sachdev
17. Lab-on-a-Chip Immunoassay for Prediction of Severe COVID-19 Disease
Harald Peter, Emily Mattig, Paul C. Guest, and Frank F. Bier
18. Multiplex Immunoassay for Prediction of Disease Severity Associated with the Cytokine Storm in COVID-19 Cases
Paul C. Guest, Mitra Abbasifard, Tannaz Jamialahmadi, Muhammed Majeed, Prashant Kesharwani, and Amirhossein Sahebkar
19. Detection of IgG Antibodies to SARS-CoV-2 and Neutralizing Capabilities Using the Luminex® xMAP® SARS-CoV-2 Multi-Antigen IgG Assay
Abbe King, Gregory King, Christy Weiss, Sherry Dunbar, and Shubhagata Das
20. Multiplex Testing of the Effect of Statins on Disease Severity Risk in COVID-19 Cases
Fatemeh Zahedipour, Paul C. Guest, Muhammed Majeed, Khalid Al-Rasadi, Tannaz Jamialahmadi, and Amirhossein Sahebkar
21. Evaluating the Effects of Curcumin on the Cytokine Storm in COVID-19 Using a Chip-Based Multiplex Analysis
Fatemeh Zahedipour, Paul C. Guest, Muhammed Majeed, Prashant Kesharwani, Tannaz Jamialahmadi, and Amirhossein Sahebkar
22. COVID-19 Detection Using the NHS Lateral Flow Test Kit
Paul C. Guest and Hassan Rahmoune
23. Evaluation Protocol for SARS-CoV-2 Serological Assays
Maemu P. Gededzha, Sarika Jugwanth, Nakampe Mampeule, Nontobeko Zwane, Anura David, Lesley Scott, Wendy Stevens, and Elizabeth S. Mayne
24. Measurement of Mitochondrial Respiration in Cryopreserved Human Peripheral Blood Mononuclear Cells (PBMCs)
Keiko Iwata, Min-Jue Xie, Paul C. Guest, Takaharu Hirai, and Hideo Matsuzazki
25. Multiplex Testing of Oxidative-Reductive Pathway in Patients with COVID-19 Disease
Paul C. Guest, Mitra Abbasifard, Tannaz Jamialahmadi, Muhammed Majeed, Prashant Kesharwani, and Amirhossein Sahebkar
26. Point-of-Care Device for Assessment of Blood Coagulation Status in COVID-19 Patients
Paul C. Guest and Hassan Rahmoune
27. COVID-19 and the Assessment of Coenzyme Q10
Nadia Turton, Robert A. Heaton, and Iain P. Hargreaves
28. Isolation and Cell Culture of Human Nasopharyngeal Cells: A Model for Testing Immune Response and Antiviral Treatment
Krist Helen Antunes and Ana Paula Duarte de Souza
29. Machine Learning Approaches to Analyze MALDI-TOF Mass Spectrometry Protein Profiles
Lucas C. Lázari, Livia Rosa-Fernandes, and Giuseppe Palmisano
30. A Deep Learning Approach to Identify Chest Computed Tomography Features for Prediction of SARS-CoV-2 Infection Outcomes
