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This volume details basic principles of experimental and computational methods for the study of microRNAs in cancer research and, therefore, provides a firm grounding for those who wish to develop further applications. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and cutting-edge, MicroRNA and Cancer: Methods and Protocols, Second Edition aims to ensure successful results in the further study of this vital field
1. Non- coding RNAs in DNA Damage Response: Opportunities for Cancer Therapeutics
Wani Arjumand, Asia Asiaf, and Shiekh Tanveer Ahmad
2. MicroRNAs in Breast Cancer: Diagnostic and Therapeutic Potential
Asia Asiaf, Shiekh Tanveer Ahmad, Wani Arjumand, and Mohammad Afzal Zargar
3. Involvement of miRNAs and Pseudogenes in Cancer
Lütfi TUTAR, Aykut ÖZGÜR, and Yusuf TUTAR
4. microRNAs Reprogram Tumor Immune Response
Wenfang Cheng and Wei Wu
5. APOBEC Gene Expression Regulated by microRNAs
Wei Cao and Wei Wu
6. MicroRNAs Changes the Landscape of Cancer Resistance
Jun Zhu and Wei Wu
7. MicroRNA, Noise, and Gene Expression Regulation
Wei Wu
PART II: Protocol Section
8. Deep Sequencing Reveals a microRNA Expression Signature in
Triple-negative Breast CancerYao-Yin Chang, Liang-Chuan Lai, Mong-Hsun Tsai, and Eric Y. Chuang
9. Detection of Plasma microRNA Signature in Osteosarcoma Patients
Wendy Allen-Rhoades and Jason T. Yustein
10. Identification of E6/E7-dependent microRNAs in HPV-positive Cancer Cells
Anja Honegger, Daniela Schilling, Holger Sültmann, Karin Hoppe-Seyler, and Felix Hoppe-Seyler
11. Combination of Anti-miRNAs Oligonucleotides with Low Amounts of Chemotherapeutic Agents for Pancreatic Cancer Therapy
Marta Passadouro and Henrique Faneca
12. Evaluation of microRNA Delivery in vivo
Rikki A. M. Brown, Kirsty L. Richardson, Felicity C. Kalinowski, Michael R. Epis, Jessica L. Horsham, Tasnuva D. Kabir, Marisa H. De Pinho, Dianne J. Beveridge, Lisa M. Stuart, Larissa C. Wintle, and Peter J. Leedman
13. Angiogenesis A
nalysis by in vitro Co-culture Assays in Transwell Chambers in Ovarian CancerAli Flores-Pérez, Dolores Gallardo Rincón, Erika Ruiz-García, Raquel Echavarria, Laurence A. Marchat, Elizbeth Álvarez-Sánchez, and César López-Camarillo
14. Application of Individual qPCR Performance Parameters for Quality Control of Circulating microRNA Data
Anna Brunet-Vega, María Elisa Quílez, María José Ramírez-Lázaro, and Sergio Lario
15. Construction of Multi-potent microRNA Sponge and its Functional Evaluation
Suhwan Chang
16. microRNA Sequencing Data Analysis Toolkits
Wei Wu
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