MicroRNA and Cancer Methods and Protocols

This volume details basic principles of experimental and computational methods for the study of microRNAs in cancer research and, therefore, provides a firm grounding for those who wish to develop further applications. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and cutting-edge, MicroRNA and Cancer: Methods and Protocols, Second Edition aims to ensure successful results in the further study of this vital field

1. Non- coding RNAs in DNA Damage Response: Opportunities for Cancer Therapeutics

Wani Arjumand, Asia Asiaf, and Shiekh Tanveer Ahmad

2. MicroRNAs in Breast Cancer: Diagnostic and Therapeutic Potential

Asia Asiaf, Shiekh Tanveer Ahmad, Wani Arjumand, and Mohammad Afzal Zargar

3. Involvement of miRNAs and Pseudogenes in Cancer

Lütfi TUTAR, Aykut ÖZGÜR, and Yusuf TUTAR

4. microRNAs Reprogram Tumor Immune Response

Wenfang Cheng and Wei Wu

5. APOBEC Gene Expression Regulated by microRNAs

Wei Cao and Wei Wu

6. MicroRNAs Changes the Landscape of Cancer Resistance

Jun Zhu and Wei Wu

7. MicroRNA, Noise, and Gene Expression Regulation

Wei Wu

PART II: Protocol Section

8. Deep Sequencing Reveals a microRNA Expression Signature in

Yao-Yin Chang, Liang-Chuan Lai, Mong-Hsun Tsai, and Eric Y. Chuang

9. Detection of Plasma microRNA Signature in Osteosarcoma Patients

Wendy Allen-Rhoades and Jason T. Yustein

10. Identification of E6/E7-dependent microRNAs in HPV-positive Cancer Cells

Anja Honegger, Daniela Schilling, Holger Sültmann, Karin Hoppe-Seyler, and Felix Hoppe-Seyler

11. Combination of Anti-miRNAs Oligonucleotides with Low Amounts of Chemotherapeutic Agents for Pancreatic Cancer Therapy

Marta Passadouro and Henrique Faneca

12. Evaluation of microRNA Delivery in vivo

Rikki A. M. Brown, Kirsty L. Richardson, Felicity C. Kalinowski, Michael R. Epis, Jessica L. Horsham, Tasnuva D. Kabir, Marisa H. De Pinho, Dianne J. Beveridge, Lisa M. Stuart, Larissa C. Wintle, and Peter J. Leedman

13. Angiogenesis A

Ali Flores-Pérez, Dolores Gallardo Rincón, Erika Ruiz-García, Raquel Echavarria, Laurence A. Marchat, Elizbeth Álvarez-Sánchez,  and César López-Camarillo

14. Application of Individual qPCR Performance Parameters for Quality Control of Circulating microRNA Data

Anna Brunet-Vega, María Elisa Quílez, María José Ramírez-Lázaro, and Sergio Lario

15. Construction of Multi-potent microRNA Sponge and its Functional Evaluation

Suhwan Chang

16. microRNA Sequencing Data Analysis Toolkits

Wei Wu