Microbial Enzymes and Biotransformations

Leading experts in enzyme manipulation describe in detail their cutting-edge techniques for the screening, evolution, production, immobilization, and application of enzymes. These readily reproducible methods can be used to improve enzyme function by directed evolution, to covalently immobilize enzymes, to microencapsulate enzymes and cells, and to manufacture enzymes for human health, nutrition, and environmental protection. Overview chapters on microorganisms as a source of metabolic and enzymatic diversity, and on the fast-moving field of enzyme biosensors are presented. Microbial Enzymes and Biotransformations offers laboratory and industrial scientists a wealth of proven enzymatic protocols that show clearly how to go from laboratory results to successful industrial applications.

Microbial Cells and Enzymes A Century of Progress.- Enzyme Biosensors.- Directed Evolution of Enzymes.- L-Glutaminase as a Therapeutic Enzyme of Microbial Origin.- Enzymatic Production of D-Amino Acids.- Screening of Microorganisms for Enzymatic Biosynthesis of Nondigestible Oligosaccharides.- Food-Grade Corynebacteria for Enzyme Production.- Enzymatic Synthesis of (S)-Phenylalanine and Related (S)-Amino Acids by Phenylalanine Dehydrogenase.- Enzymes in Modern Detergents.- Microbial Proteases.- Proteases Produced by Halophilic Bacteria and Archaea.- Microbial Pectinases.- Expression of Microbial Phytases in Yeast Systems and Characterization of the Recombinant Enzymes.- Glucose Dehydrogenase for the Regeneration of NADPH and NADH.- Acetate Kinase From Methanosarcina thermophila, a Key Enzyme for Methanogenesis.- Immobilization of Enzymes by Covalent Attachment.- Preparation of an Industrial Biocatalyst of Penicillin G Acylase on Sepabeads.- Methods for Bioencapsulation of Enzymes and Cells.