Metaproteomics Methods and Protocols

This volume provides references for methods about the proteomics of microbial communities, also called metaproteomics. Chapters guide readers first through specific protein extractions from different environments and/or ecological niches crowded by heterogeneous microbial communities, then deepening the possible complete metaproteomic workflows in several situations or conditions. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.

Authoritative and cutting-edge, Metaproteomics: Methods and Protocols aims to ensure successful results in the further study of this vital field.

Extraction of proteins from marine microbial communities sampled by lab filters.- Extraction of proteins from municipal wastewater and activated sludge.- Extraction of proteins from fermented food.- Extraction of proteins from soil.- Extraction of proteins from green tissues of plants and phyllosphere.- Extraction of proteins from microbiome of livestocks.- A complete metaproteomic workflow for Arabidopsis roots inoculated by synthetic bacteria.- Proteomics for microalgae extracts by high-resolution mass spectrometry.- Metaproteomic analysis of microbial communities affecting fishery products.- Metaproteomic analysis of biogas plants: a complete workflow from lab to bioinformatics.- Metaproteomic analysis of fecal samples from human subjects and rodent models.- Metaproteomics of gut fungi in gnotobiotic mice.- Fungal secretomics through iTRAQ-based analysis.- Label-free quantitative proteomics of oral microbial communities.- Metaproteomics for co infections in the upper respiratory tract the case of COVID-19.- Bioinformatic workflows for metaproteomics.