Metabolic Profiling Methods and Protocols

This second edition volume expands on the previous edition with new chapters and updated discussions on the latest advancements in the development and practice of metabolic phenotyping. The chapters in this book cover topics such as quality control in untargeted metabolic phenotyping; implementation of quality assurances processes; careful bio- and chemoinformatic data analysis; using reversed-phase and ion-pair LC-MS; quantitative lipid analysis using supercritical fluid chromatography (SFC-MS) and the targeted  determination of metanephrines in urine via LC-MS/MS. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Thorough and comprehensive, Metabolic Profiling: Methods and Protocols, Second Edition is a valuable resource for all researchers who are interested in incorporating best practices in metabolic phenotyping and learning more about this rapidly advancing field.

Metabolic Profiling: A Perspective on the Current Status, Challenges, and Future Directions.- Quality Assurance in Metabolomics and Metabolic Profiling.- Quality Control and Validation Issues in LC-MS-Based Metabolomics.- Bio- and Chemoinformatics Approaches for Metabolomics Data Analysis.- Data Treatment for LC-MS Untargeted Analysis.- Untargeted Metabolic Phenotyping by LC-MS.- Quantitative Lipidomics of Biological Samples using Supercritical Fluid Chromatography Mass Spectrometry.- Rat Fecal Metabolomics-Based Analysis.- Ion Pair Chromatography for Endogenous Metabolite LC-MS Analysis in Tissue Samples Following HGH Resolution Untargeted Acquisition.- HILIC-MS/MS Multi-Targeted Method for Metabolomics Applications.- A Protocol for GC-MS Profiling of Chiral Secondary Amino Acids.- UHPLC-TIMS-PASEF-MS for Lipidomics: From Theory to Practice.- Advanced LC-IMS-MS Protocol for Holistic Metabolite analysis in Wine and Grape Samples.- Analysis of Urinary Metanephrines Using Liquid Chromatography Tandem Mass Spectrometry.- Two-Phase Extraction for Comprehensive Analysis of the Plant Metabolome by NMR.- Olive Fruit Phenolic Profiling using High Resolution-Magic Angle Spinning (HR-MAS) Solid State NMR Spectroscopy.