Mass Spectrometry Imaging of Small Molecules Methods and Protocols

This volume presents updated methods and new developments in the field of mass spectrometry imaging. Chapters guide readers through four parts covering imaging, software,  data analysis, new instrumentation, and new methodological approaches. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

 Authoritative and cutting-edge, Mass Spectrometry Imaging of Small Molecules aims to be a useful practical guide to researchers to help further their study in this field. 

Gas-phase Ion/Ion Reactions for Lipid Identification in Biological Tissue Sections.- MALDI-2 and t-MALDI-2 Mass Spectrometry Imaging.- Ambient Mass Spectrometry Imaging of Small Molecules from Cells and Tissues.- Single-Cell Metabolomics with Rapid Determination of Chemical Formulas from Isotopic Fine Structures.- TOF-SIMS Imaging of Biological Tissue Sections and Structural Determination using Tandem MS.- Mass Spectrometry Imaging of Biological Tissues by Laser Desorption Ionization from Silicon Nanopost Arrays.- Metal-assisted Laser Desorption Ionization Imaging Mass Spectrometry.- Mass Spectrometry Imaging of Metabolites by Nanostructure Initiator Mass Spectrometry with Fluorinated Gold Nanoparticles.- Imaging of Plant Hormones with Nanoparticle-Assisted Laser Desorption/Ionization Mass Spectrometry.- Nanosecond Photochemical Reaction (nsPCR) for Enhanced Mass Spectrometric Identification, Quantification and Visualization of Metabolites and Neuropeptides.- Extraction and Up-Concentration of Adsorbates from Precisely Defined Area for Quantitative MALDI Mass Spectrometry Imaging.- The Development and Application of DropletProbe-Mass Spectrometry for Examining Biodistribution of Therapeutics.- Quantitative MS imaging with Liquid Microjunction Surface Sampling.- Enhancing Metabolite Coverage for Matrix-Assisted Laser Desorption/Ionization-Mass Spectrometry Imaging Through Multiple On-Tissue Chemical Derivatizations.- Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry Imaging of Glycogen in Situ.- MALDI Mass Spectrometry Imaging of Lipids on Free-Floating Brain Sections and Immunohistochemically Colocalized Markers of Neurodegeneration.- Silver-doped Nano-DESI MSI for Increased Specificity and Sensitivity of Alkenes.- Extract Metabolomic Information from Mass Spectrometry Images using Advanced Data Analysis.