Mass Spectrometry for Metabolomics

This detailed volume provides a comprehensive overview of state-of-the-art metabolomics methods based on mass spectrometry (MS), and their application in food, nutrition, and biomedical research. The chapters assembled herein cover hot topics related to sample preparation, chromatographic and electrophoretic separation, MS-based analysis, as well as data processing and analysis. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Comprehensive and High-Throughput Method for Quantitative Fingerprinting of Phenolic Compounds.- Determination of Volatile Metabolites in Vinegars by Solid Phase Microextraction-Gas Chromatography-Mass Spectrometry (SPME-GC-MS).- Determination of Volatile Metabolites in Vinegars by Stir Bar Sorptive Extraction-Gas Chromatography-Mass Spectrometry (SBSE-GC-MS).- Discovery of Food Intake Biomarkers Using Metabolomics.- Metabolomic Characterization of the Antiproliferative Activity of Bioactive Compounds from Fruit By-Products Against Colon Cancer Cells.- Untargeted Metabolomics by Liquid Chromatography-Mass Spectrometry in Biomedical Research.- Untargeted Metabolomics for Disease-Specific Signatures.- Metabolomics Analysis of Blood, Urine, and Saliva Samples Based on Capillary Electrophoresis-Mass Spectrometry.- Capillary Electrophoresis-Mass Spectrometry for the Direct Analysis of Metabolites in Highly Saline Samples Using In-Capillary Preconcentration.- Chemical Derivatization to Enhance Capillary Electrophoresis Mass Spectrometric Analysis of Acidic Metabolites in Mammalian Cells.- Untargeted Metabolomics Based on Liquid Chromatography-Mass Spectrometry for the Analysis of Plasma and Erythrocyte Samples in Childhood Obesity.- Characterization of the Plasmatic and Erythroid Multi-Elemental Biodistribution in Childhood Obesity Using a High-Throughput Method for Size-Fractionation of Metal Species.- Analysis of Brain Metabolites Using Two Complementary Ultra-High Performance Liquid Chromatography Mass Spectrometry Methods.- Analysis of Cholesterol from the Liver Using Gas Chromatography-Mass Spectrometry.- Metabolomics Analysis of Aqueous Humor Based on Liquid Chromatography-Mass Spectrometry.- Analyses and Localization of Serotonin and L-DOPA in Ocular Tissues by Imaging Mass Spectrometry.- Isobaric Incorporation of C13-Histidine for the Assessment of Remyelination.- Isolation and Lipidomic Screening of Human Milk Extracellular Vesicles.- Mass Spectrometry-Based Untargeted Metabolomics and Lipidomics Platforms to Analyze Cell Culture Extracts.- Data Processing and Analysis in Mass Spectrometry-Based Metabolomics.- Data Processing and Analysis in Liquid Chromatography-Mass Spectrometry-Based Targeted Metabolomics.- Instrument-Agnostizing Methodology for Liquid Chromatography-Mass Spectrometry Systems.