Long Non-Coding RNAs Methods and Protocols

This second edition provides a broad spectrum of methods used in long non-coding RNAs (lncRNA) research, ranging from computational annotation of lncRNA genes to molecular and cellular analyses of the function of individual lncRNA. Chapters guide readers through studies used to circular RNAs, RNA splicing, and findings on lncRNA in human diseases. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

 

Authoritative and cutting-edge, Long Non-Coding RNAs: Methods and Protocols,Second Edition aims to ensure successful results in the further study of this vital field.

LncRNA pulldown Combined with Mass Spectrometry to Identify the Novel lncRNA-associated Proteins.- Cross-Linking Immunoprecipitation and qPCR (CLIP-qPCR) Analysis to Map Interactions of Long Noncoding RNAs with Canonical and Non-canonical RNA-binding Proteins.- Characterization of Long Non-coding RNA Associated Proteins by RNA-immunoprecipitation.- Isolation of Protein Complexes Associated with Long Non-Coding RNAs.- Detection of Long Noncoding RNA Expression by Real-time PCR.- Profiling Long Non-coding RNA expression using Custom-Designed Microarray.- Long Non-coding RNA Expression Profiling using Arraystar LncRNA Microarrays.- Nuclear RNA Isolation and Sequencing.- Capturing Endogenous Long Noncoding RNAs and Their Binding Proteins Using Chromatin Isolation by RNA Purification.- Purification and Structural Characterization of the Long Noncoding RNAs.- Simultaneous RNA-DNA FISH.- Highly Resolved Detection of Long Non-coding RNAsIn Situ.- Detection of Long Non-coding RNA Expression by Non-radioactive Northern Blots.- Assessment of in vivo siRNA Delivery in Cancer Mouse Models.- Genetic Editing of Long Non-Coding RNA Using CRISPR/Cas9 Technology.- Characterization of Circular RNAs.- Identification of circRNA-Interacting Proteins by Affinity Pulldown.- Exploration to Circular RNA Interactomes by RNA Pull-Down Method.- Methods for Characterization of Alternative RNA Splicing.- Computational analysis of lncRNA from cDNA sequences.- Characterizing miRNA-lncRNA interplay.- Illuminating lncRNA Function through Target Prediction.- Long Noncoding RNAs: An Overview.