Investigations of Early Nutrition Effects on Long-Term Health Methods and Applications

This volume contains several reviews in the field as well as the step-by-step use of targeted and global approaches within the areas of genomics, epigenetics, proteomics, transcriptomics and metabolomics which aim to address this dilemma and to help pinpoint new treatment strategies. Chapters detail the generation of several models and methods for assessing health and provide researchers potential approaches for reversing or minimizing effects of disease. In addition, important information on disease mechanisms is provided, as each method is described in the context of a specific disease or therapeutic area. Written in the highly successful Methods in Molecular Biology series format, the chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and practical, Investigations of Early Nutrition Effects on Long-Term Health: Methods and Applications aim is to provide insights into the latest ideas and technologies .enabling progress in this field.

Part I: Reviews

1.                  Nutritional Programming Effects on Development of Metabolic Disorders in Later Life

Thomas Prates Ong and Paul C. Guest

2.                  Effects of Prenatal Nutrition and the Role of the Placenta in Health and Disease

Leslie Myatt and Kent L Thornburg

3.                  Developmental Origins of Stress and Psychiatric Disorders

Francesca L Guest and Paul C. Guest

4.                  Proteomic Studies of Psychiatric Disorders

Paul C. Guest

5.                  Developmental Origins of Breast Cancer: A Paternal Perspective

Camile Castilho Fontelles, Raquel Santana da Cruz, Leena Hilakivi-Clarke, Sonia de Assis, and Thomas Prates Ong

6.                  Point-of-care Testing and Personalized Medicine for Metabolic Disorders

Francesca L. Guest and Paul C. Guest

7.                  Pregnancy and Lactation a Window of Opportunity to Improve Individual Health

Guadalupe L. Rodríguez-González, Diana C Castro-Rodríguez, and Elena Zambrano

8.                  Utility of Small Animal Models of Developmental Programming

Clare M Reynolds and Mark H Vickers

Part II: Protocols

9.                  Generation of Maternal Obesity Models in Studies of Developmental Programming in Rodents

Paul D Taylor, Phillippa A Matthews, Imran Y Khan, Douglas Rees, NozomiItani, Lucilla Poston

10.              Generation of the Maternal Low Protein Rat Model for Studies of Metabolic Disorders

Dan Ma, Susan E. Ozanne, and Paul C. Guest

11.              Investigation of Paternal Programming of Breast Cancer Risk in Female Offspring in Rodent Models

Camile Castilho Fontelles, Raquel Santana da Cruz, Leena Hilakivi-Clarke, Sonia de Assis, and Thomas Prates Ong

12.              Studies of Isolated Peripheral Blood Cells as a Model of Immune Dysfunction

Hassan Rahmoune and Paul C. Guest

13.              Studies of a Neuronal Cell Line as a Model of Psychiatric Disorders

Keiko Iwata

14.              Assessment of Placental Transport Function in Studies of Disease Programming

Amanda N. Sferruzzi-Perri

15.              Assessment of Fatty Liver in Models of Disease Programming

Kimberley D Bruce and Karen R Jonscher

16.              Capillary Blood Sampling from the Finger

Kieron Rooney

17.              Physical Activity Assessment in Clinical Studies of Substance use Disorder

Rhiannon Dowla, Bridin Murnion, Kia Roberts, Jonathan Freeston, and Kieron Rooney

Derek Tran

19.              Cardiovascular Assessment in Human Research

20.              Nutritional Programming Effects on the Immune System

Donald B. Palmer

Lucas Carminatti Pantaleão and Susan E. Ozanne

22.              Pulse-chase Biosynthetic Radiolabelling of Pancreatic Islets to Measure Beta Cell Function

Paul C. Guest

23.              Rapid and Easy Protocol for Quantification of Next Generation Sequencing Libraries

Steve F.C. Hawkins and Paul C. Guest

Jane L. Tarry-Adkins and Susan E. Ozanne

25.              Qualitative and quantitative NMR Approaches in Blood Serum Lipidomics

Banny Silva Barbosa, Lucas Gelain Martins, TássiaB. B. C. Costa, Guilherme Cruz, and LjubicaTasic

26.              In vivo Electrical Stimulation for the Assessment of Skeletal Muscle Contractile Function in Murine Models

Kaio F. Vitzel, Marco A. Fortes, Gabriel N. Marzuca-Nassr, Maria V.M. Scervino, Carlos H. Pinheiro, Leonardo R. Silveira, and Rui Curi

27.              Experimental Model of Skeletal Muscle Laceration in Rats

28.              Neuropsychiatric Sequelae of Early Nutritional Modifications: A Beginner’s Guide to Behavioural Analysis

Ann-Katrin Kraeuter, Paul C. Guest, and Zoltán Sarnyai

Dan Ma and Paul C. Guest

30.              2D-DIGE Analysis of Eye Lens Proteins as a Measure of Cataract Formation

Paul C. Guest

31.              Mass Spectrometry Profiling of Pituitary Glands

Divya Krishnamurthy, Hassan Rahmoune, and Paul C. Guest

32.              Multiplex Immunoassay Profiling of Hormones Involved in Metabolic Regulation

Laurie Stephen and Paul C. Guest

33.              Time Resolved Fluorescence Assays for Quantification of Insulin Precursors in Plasma and Serum

Kevin Taylor, Ian Halsall, Paul C. Guest, and Keith Burling

34.              Identification of Neural Stem Cell Biomarkers by Isobaric Tagging for Relative and Ab