Intracellular Pathogens Methods and Protocols

This detailed volume presents cutting-edge approaches to study infection, immunity, and intervention strategies against intracellular pathogens. The book explores areas such as how intracellular pathogens infect and survive inside the phagocytes, immunopathological features of infection, evasion of host-induced immune responses, cellular crosstalk in the presence of intracellular pathogens, host innate and adaptive immunity, and much more. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Intracellular Pathogens: Infection, Immunity, and Intervention.- Methods for Genomic Epidemiology of Bacterial Pathogens: Example Salmonella.- Phagocytosis.- Large Scale Culture and Plasmid Preparation Procedure for Low-Yield Bacmids Containing Full-Length SARS-CoV-2 cDNAs.- Metatranscriptomic RNA-Seq Data Analysis of Virus-Infected Host Cells.- Metabolic Analysis of Intracellular Pathogenic Bacteria Using NMR.- Quantitative Proteomics of the Intracellular Bacterial Pathogen Salmonella enterica Serovar Typhimurium.- Median Tissue Culture Infectious Dose 50 (TCID₅₀) Assay to Determine Infectivity of Cytopathic Viruses.- Applications of Quantitative PCR (qPCR) in Studies of Virus-Host Interactions.- Establishing Air-Liquid Interface (ALI) Airway Culture Models for Infectious Disease Research.- Pig Acute Phase Proteins as Non-Antibody Systemic Biomarkers of Intracellular Infections.- Quantification of Mycobacterium tuberculosis Growth in Cell-Based Infection Assays by Time-Lapse Fluorescence Microscopy.- Intravital Microscopy for Imaging and Live Cell Tracking of Alveolar Macrophages in Real Time.- Recent Advancements in Radiopharmaceuticals for Infection Imaging.- Bacteriophages Isolation and Efficacy Testing.- Production, Isolation, and Detection of Poly IC in Extracellular Vesicles from U937 Cells.- In Silico Tools for Predicting Novel Epitopes.- In Situ Characterization of Human Follicular Helper CD4 T Cells.- Identification and Characterization of Antigen-Specific T-Cells in Viral Infections.- Evaluating Cationic Nanoparticles as Carriers of Antiviral Nucleic Acids.- RNA-Based Vaccines and Therapeutics Against Intracellular Pathogens.