Immune Homeostasis Methods and Protocols

This volume discusses different aspects of immune homeostasis regulation and its dysregulation during various diseases, including infections, cancers, neurodegenerative diseases, autoinflammation, autoimmune diseases, and immunodeficiency, along with immunological, genetic, cell biology, and molecular biology techniques designed to understand immune homeostasis at different stages. The chapters in this book cover topics such as T cell subsets; monocyte-myofibroblast immunomodulation in a 3D co-culture system; human peripheral blood mononuclear cells in patients with neurodegenerative diseases; and CRISPR-Cas9 induced gene-editing in primary human monocytes. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Cutting-edge and comprehensive, Immune Homeostasis: Methods and Protocols is a valuable resource for both novice and experts researchers interested in learning more about this important and developing field.  

Immune Homeostasis: A Novel Example of Teamwork.- Immune Homeostasis Maintenance through Advanced Immune Therapeutics to Target Atherosclerosis.- T Cell Subsets and Immune Homeostasis.- Post-Transcriptional Events Orchestrate Immune Homeostasis of CD8+T Cells.- A Robust In Vitro Culture Model and Generation of Memory Monocytes.- A Robust In Vitro Co-Culture Model for Studying the Intercellular Communication of Neutrophils.- Studying Monocyte-Myofibroblast Immunomodulation in a 3D Co-Culture System.- Endothelial Mitochondria-Associated Membranes (MAMs) Isolation by Percoll Step Gradients.- Analysis of Human Peripheral Blood Mononuclear Cells in Patients with Neurodegenerative Diseases.- Modulation and Determination of the Status of Inflammasomes in Leishmania-Infected Macrophages.- Fluid-Dynamic Culture of Tumor and Immune Cells for More Predictive Infiltration Studies and Immunotherapy Drug Screening.- Isolation and Single-Cell Transcriptomic Analysis of Murine Regulatory B Cells.- Isolation and Culture of Mouse Primary Microglia and Oligodendrocyte Precursor Cells.- Phenotypic and Functional Characterization of Memory CD4+ and CD8+ T Cells after Antigenic Stimulation.- CRISPR-Cas9 Induced Gene-Editing in Primary Human Monocytes.- Employing CRISPR-Cas9 to Enhance T Cell Effector Function.- Assessing Antigen-Specific T Cell Responses through IFN- Enzyme-Linked Immune Absorbent Spot (ELISpot).