Imaging Mass Spectrometry Methods and Protocols

This second edition details new and updated chapters on key methodologies and breakthroughs in the mass spectrometry imaging (MSI) field. Chapters guide readers through nano-Desorption Electrospray Ionisation (nDESI), Matrix Assisted Laser Desorption Ionisation-2 (MALDI-2), Laser Ablation - Inductively Coupled Plasma-Mass Spectrometry (LA-ICP-MS) ,Imaging Mass Cytometry (IMC) with a variety of diverse samples including eye tissue, crop analysis, 3D cell culture models, and counterfeit goods analysis. Written in the format of the highly successful Methods in Molecular Biology series, each chapter includes an introduction to the topic, lists necessary materials and reagents, includes tips on troubleshooting and known pitfalls, and step-by-step, readily reproducible protocols.

 

Authoritative and cutting-edge, Imaging Mass Spectrometry: Methods and Protocols, Second Edition aims to be a useful and practical guide to new researchersand experts looking to expand their knowledge. 

MALDI and Trace Metal Analysis in Age Related Macular Degeneration.- HistoSnap: a novel software tool to extract m/z-specific images from large MSHC datasets.- Spatially resolved quantitation of drug in skin equivalents using Mass Spectrometry Imaging (MSI).- Update DESI Mass Spectrometry Imaging (MSI).- Update Liquid Extraction Surface Analysis Mass Spectrometry Imaging of Denatured Intact Proteins.- MALDI MS imaging of cucumbers.- The adaptation of the QV600 LLI Milli-Fluidics System to house ex vivo gastrointestinal tissue suitable for drug absorption and permeation studies, utilising MALDI-MSI and LC-MS/MS.- Ambient Mass Spectrometry Imaging by Water-Assisted Laser Desorption Ionization For Ex Vivo And In Vivo Applications.- Cytological cytospin preparation for the spatial proteomics analysis of thyroid nodules using MALDI-MSI.- Matrix effects free imaging of thin tissue sections using pneumatically assisted nano-DESI MSI.- Laser Ablation Inductively Coupled Plasma Mass Spectrometry Imaging of Plant Materials.- Sample Preparation for Metabolite Detection in Mass Spectrometry Imaging.- Multimodal Mass Spectrometry Imaging of an Aggregated 3D Cell Culture Model.- Visualization of Small Intact Proteins in Breast Cancer FFPE tissue.- Negative Ion-mode N-glycan Mass Spectrometry Imaging by MALDI-2-TOF-MS.- MS1-based data analysis approaches for FFPE tissue imaging of endogenous peptide ions by mass spectrometry histochemistry (MSHC).- Mass Spectrometry Imaging: The Next Five Years.