Imaging Flow Cytometry Methods and Protocols

This detailed volume for the first time explores techniques and protocols involving quantitative imaging flow cytometry (IFC), which has revolutionized our ability to analyze cells, cellular clusters, and populations in a remarkable fashion. Beginning with an introduction to technology, the book continues with sections addressing protocols for studies on the cell nucleus, nucleic acids, and FISH techniques using an IFC instrument, immune response analysis and drug screening, IFC protocols for apoptosis and cell death analysis, as well as morphological analysis and the identification of rare cells.  Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and practical, Imaging Flow Cytometry: Methods and Protocols will be a critical source for all laboratories seeking to implement IFC in their research studies.

Part I: General

1. Quantitative Functional Morphology by Imaging Flow Cytometry

            Ivan A. Vorobjev and Natasha S. Barteneva

2. Principles of Amnis Imaging Flow Cytometry

            David A. Basiji

3. Ultrafast Microfluidic Cellular Imaging by Optical Time-Stretch

            Andy K.S. Lau, Terence T.W. Wong, Ho Cheung Shum, Kenneth K.Y. Wong, and Kevin K. Tsia

4. Applications of Imaging Flow Cytometry for Microalgae

            Mark Hildebrand, Aubrey Davis, Raffaela Abbriano, Haley R. Pugsley, Jesse C. Traller, Sarah R. Smith, Roshan P. Shrestha, Orna Cook, Eva Sanchez, Kalpana Manandhar-Shrestha, and Benjamin Alderete

Part II: IFC in Detection of Nuclei and Nucleic Acids

5. The Analysis of Cell Cycle, Proliferation, and Asymmetric Cell Division by Imaging Flow Cytometry

            Andrew Filby, William Day, Sukhveer Purewal, and Nuria Martinez-Martin

6. Quantitation of Chromosome Damage by Imaging Flow Cytometry

            Lindsay A. Beaton-Green and Ruth C. Wilkins

7. Fluorescent In Situ Hybridization In Suspension by Imaging Flow Cytometry

            Orla Maguire, Paul K. Wallace, and Hans Minderman

8. Analysis of Nucleo-Cytoplasmic Protein Shuttling by Imaging Flow Cytometry

            Elizaveta Fasler-Kan, Yeldar Baiken, Ivan Vorobjev, and Natasha S. Barteneva

9. Usin

            Adam S. Venable, Andrea L. Henning, Eric A. Prado, and Brian K. McFarlin

Part III: IFC in Immune Response and Drug Screening

10. Multiparametric Characterization of Human T-Cell Immune Synapses by InFlow Microscopy

            Guido H. Wabnitz and Yvonne Samstag

11. Studying T Cells N-Glycosylation by Imaging Flow Cytometry

            Ana M. Dias, Catarina R. Almeida, Celso A. Reis, and Salomé S. Pinho

12. Assessment of Granulocyte Subset Activation: New Information from Image-Based Flow Cytometry

            Brian K. McFarlin, Eric A. Prado, Adam S. Venable, Andrea L. Henning, and Randall R. Williams

13. Using Image-Based Flow Cytometry to Assess Monocyte Oxidized LDL Phagocytosis Capacity

            Andrea L. Henning, Adam S. Venable, Eric A. Prado, and Brian K. McFarlin

14. Screening for Drugs Against the Plasmodium falciparum Digestive Vacuole by Imaging Flow Cytometry

            Yan Quan Lee, Brian E.Hall, and Kevin S.W. Tan

Part IV: IFC in Studies of Cell Death and Apoptosis

15. Accurate Assessment of Cell Death by Imaging Flow Cytometry

            Aja M. Rieger and Daniel R. Barreda

16. Temporal Heterogeneity

            Ivan A. Vorobjev and Natasha S. Barteneva

Part V: Imaging Cytometry in Cell Classification and Morphological Analysis

17. FlowCAM: Quantification and Classification of Phytoplankton by Imaging Flow Cytometry

            Nicole J. Poulton

18. Detection and Characterization of Rare Circulating Endothelial Cells by Imaging Flow Cytometry

            Leigh Samsel and J. Philip McCoy, Jr.

19. Imaging Flow Cytometric Analysis of Primary Bone Marrow Megakaryocytes

            Lisa M. Niswander, James Palis, and Kathleen E. McGrath

20. Sickle Cell Imaging Flow Cytometry Assay (SIFCA)

            Kleber Y. Fertrin, Leigh Samsel, Eduard J. van Beers, Laurel Mendelsohn, Gregory J. Kato, and J. Philip McCoy