Ice Binding Proteins Methods and Protocols

This volume provides methods to study ice-binding proteins (IBPs), and applications involving these proteins. Chapters are divided into three parts describing identifying, isolating, and purifying ice-binding proteins, characterize both IBPs and ice-nucleating proteins (INPs), and synthesize mimics of IBPs, as well as applications involving these proteins. Written in the format of the highly successful Methods in Molecular Biology series, each chapter includes an introduction to the topic, lists necessary materials and reagents, includes tips on troubleshooting and known pitfalls, and step-by-step, readily reproducible protocols.   

Authoritative and cutting-edge, Ice Binding Proteins: Methods and Protocols aims to provide both experimental and simulations protocols to help new researchers, break-the-ice, and enter this exciting field, while also supporting established researchers broaden the scope of theirinvestigations. 

Identifying Ice-Binding Proteins in Nature.- Ice-shell purification of ice-active compounds.- Ice Isn’t the Only Crystal in Town: Structure Determination of Ice-Binding Proteins Via X-ray Crystallography.- Large Scale Purification of Natural Ice Binding Proteins by the Falling Water Ice Purification Method.- The Nanoliter Osmometer - Thermal Hysteresis Measurement.- Evaluation of ice recrystallization inhibition of ice-binding proteins by monitoring specific ice crystals.- Measurement of Ice Nucleation Activity of Biological Samples.- Investigating the interaction between ice-binding proteins and ice surfaces using microfluidicdevices and cold stages.- Quantification of the ice nucleation activity of ice-binding proteins using a microliter droplet freezing experiment.- Measurement of Ice-Binding Protein Activity in Highly Alkaline Environments.- Measurement of ice binding proteins inhibition of non-ice crystal growth.- Divergent Mechanisms of Ice Growth Inhibition by Antifreeze Proteins.- Multiscale Molecular Dynamics Simulations of Ice-Binding Proteins.- Synthesis of polymer mimics of ice-binding proteins.- Generating ice-binding protein-polymer bioconjugates.- Analysis of ice binding protein evolution.