Germ Cell Development Methods and Protocols

Isolation and Purification of Viable PGCs from Mouse Embryos.- Purification and Transfection Methods of Chicken Primordial Germ Cells.- Isolation and In Vitro Propagation of Human Spermatogonial Stem Cells.- Purification by STA-PUT Technique of Male Germ Cells from Single Mouse and RNA-Extraction for Transcriptomic Analysis.- Isolation of Mouse Germ Cells by FACS Using Hoechst 33342 and SYTO16 Double Staining.- Isolation and In Vitro Culture of Germ Cells and Sertoli Cells from Human Fetal Testis.- Human Primordial Germ Cell-Like Cell Induction from Pluripotent Stem Cells by SOX17 and PRDM1 Expression.- Induction of Primordial Germ Cell-Like cells from Rat Pluripotent Stem Cells.- Induction of Meiotic Initiation in Long-Term Mouse Spermatogonial Stem Cells Under Retinoid Acid and Nutrient Restriction Conditions.- Lipofection-Based Delivery of CRISPR/Cas9 Ribonucleoprotein for Gene Editing in Male Germline Stem Cells.- Mouse In Vitro Spermatogenesis on 3D Bioprinted Scaffolds.-Histological and Cytological Techniques to Study Perinatal Mouse Ovaries and Oocytes.- Method of Isolation and In Vitro Culture of Primordial Follicles in Bovine Animal Model.- Generation of iPSC Cell Lines from Patients with Sex Chromosome Aneuploidies.- Data Analysis Pipeline for scRNA-Seq Experiments to Study Early Oogenesis.- Mapping Meiotic DNA Breaks: Two Fully-Automated Pipelines to Analyze Single-Strand DNA Sequencing Data, hotSSDS and hotSSDSextra.- “MeiQuant”: An Integrated Tool for Analyzing Meiotic Prophase I Spread Images.