Genotype Phenotype Coupling Methods and Protocols

Screening, Post-SELEX Optimization and Application of DNA Aptamers Specific for Tobramycin.- Affitins: Ribosome Display for Selection of Aho7c-based Affinity Proteins.- cDNA Display: A Stable and Simple Genotype–phenotype Coupling Using a Cell-free Translation System.- cDNA Display of Disulfide-containing Peptide Library and in vitro Evolution.- Rapid Antigen and Antibody-like Molecule Discovery by Staphylococcal Surface Display.- Restriction-free Construction of a Phage-presented Very Short Macrocyclic Peptide Library.- In Vitro Maturation of a Humanized Shark VNAR Domain to Improve Its Biophysical Properties.- Antibody Phage Display: Antibody Selection in Solution Using Biotinylated Antigens.- Assessing Antibody Specificity in Human Serum Using Deep Sequence-coupled Biopanning.- Isolation of Antigen-specific VHH Single Domain Antibodies by Combining Animal Immunization with Yeast Surface Display.- Selection and Characterization of Anti-idiotypic Shark Antibody Domains.-Simplifying the Detection of Surface Presentation Levels in Yeast Surface Display by Intracellular tGFP Expression.- Methods for Construction of Yeast Display Libraries of Four-Domain T-cell Receptors.- Isolation of Tailor-made Antibody Fragments from Yeast-displayed B-Cell Receptor Repertoires by Multiparameter Fluorescence-Activated Cell Sorting.- Isolation of Anti-Hapten Antibodies by Fluorescence-Activated Cell Sorting of Yeast-Displayed B-Cell Receptor Gene Repertoires.- Rapid Generation of Chicken Immune Libraries for Yeast Surface Display.- Ligand Engineering via Yeast Surface Display and Adherent Cell Panning.- Simultaneous Soluble Secretion and Surface Display of Proteins in Saccharomyces Cerevisiae Using Inefficient Ribosomal Skipping.- Chemical Modification of the Yeast Cell Surface Allows the Switch between Display and Soluble Secretion of Full-length Antibodies.- Advanced Establishment of Stable Recombinant Human Suspension Cell Lines Using Geno-Phenotype Coupling Transposon Vectors.- Mammalian Surface Display Screening of Diverse Cystine-Dense Peptide Libraries for Difficult to Drug Targets.- Engineering Antibodies on the Surface of CHO Cells.- Single B Cell Cloning and Production of Rabbit Monoclonal Antibodies.